Identification of an E-box motif responsible for the expression of jasmonic acid-induced chitinase gene OsChia4a in rice
Identification of an E-box motif responsible for the expression of jasmonic acid-induced chitinase gene OsChia4a in rice
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DOI：
10.1016/j.jplph.2011.12.008
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发表时间：
2012-04-15
影响因子：
4.3
通讯作者：
Okada, Kazunori
中科院分区：
文献类型：
--
作者：
Miyamoto, Koji;Shimizu, Takafumi;Okada, Kazunori
已知植物激素茉莉酸（JA）参与针对病原体的多种防御反应，其中包括产生病程相关（PR）蛋白。为了研究茉莉酸诱导水稻防卫反应的机制，我们对一个几丁质酶基因OsChia 4a进行了转录组分析，该基因是茉莉酸诱导水稻防卫反应最强的基因之一。OsChia 4a重组蛋白对稻瘟病菌孢子萌发和菌丝生长具有抑制作用。OsChia 4a的启动子分析表明，ATG翻译起始位点上游-515 ~-265 bp的区域是JA应答所必需的。随后的突变分析表明，在这个区域的E盒（CANNTG）作为一个JA响应顺式元件。这些结果表明，一个基本的螺旋-环-螺旋转录因子可能参与调控OsChia 4a的表达在JA依赖的方式。(C)2011年Elsevier GmbH。All rights reserved.
The plant hormone jasmonic acid (JA) is known to be involved in multiple defence responses against pathogens, which include the production of pathogenesis-related (PR) proteins. In order to investigate the induction mechanism of the rice defence responses by JA, we performed transcriptome analyses and focused on a chitinase gene, OsChia4a, which was identified to be one of the highest JA-inductive genes. The recombinant protein of His-tagged OsChia4a exhibited an inhibitory effect against the spore germination and hyphal growth of Magnaporthe oryzae. The promoter analysis of OsChia4a revealed that the region from -515 bp to -265 bp upstream of the ATG translation initiation site was required for the responsiveness to JA. A subsequent mutation analysis indicated that an E-box (CANNTG) in this region act as a JA-responsive cis element. These results imply that a basic helix-loop-helix transcription factor is likely to be involved in the regulation of the OsChia4a expression in a JA-dependent manner. (C) 2011 Elsevier GmbH. All rights reserved.