RANDOM AMPLIFIED HYBRIDIZATION MICROSATELLITES (RAHM) - ISOLATION OF A NEW CLASS OF MICROSATELLITE-CONTAINING DNA CLONES
RANDOM AMPLIFIED HYBRIDIZATION MICROSATELLITES (RAHM) - ISOLATION OF A NEW CLASS OF MICROSATELLITE-CONTAINING DNA CLONES
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DOI：
10.1093/nar/23.18.3802
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发表时间：
1995-09-25
影响因子：
14.9
通讯作者：
CELLINI, F
中科院分区：
文献类型：
--
作者：
CIFARELLI, RA;GALLITELLI, M;CELLINI, F
微卫星重复序列广泛存在于真核生物基因组(1，2)中，分布于整个基因组DNA中。已经为包括植物(5-7)在内的各种生物(3、4)开发了微辉石标记。微卫星序列两侧的区域可在使用微卫星引物的聚合酶链式反应中扩增，从而提供共显性序列标签位点(STS)标记。然而，开发这种分子标记的努力是相当费力的，需要建立文库、筛选和克隆测序。几个小组已经开发了使用PCR检测(8)和分离侧翼序列(9、10)的替代方法。随机扩增多态性DNA(随机扩增多态性DNA)标记易于开发，检测成本较低，但通常是遗传显性的，具有较低的多态程度。
Microsatellite repeats are widely present in eukaryotic genomes (1, 2) and are interspersed throughout the genomic DNA. Microsa-tellite markers have been developed for a variety of organisms (3, 4), including plants (5-7). The regions flanking microsatellite sequences can be amplified in a PCR reaction using microsatellite primers, thus providing codominant sequence tagged sites (STS) markers. However, efforts to develop such molecularmarkers are quite laborious, requiring library construction, screening, and sequencing of clones. Several groups have developed alternative methods for detection (8) and isolation of flanking sequences (9, 10) using PCR. Random amplified polymorphic DNA (RAPD) markers are easy to develop and less expensive to assay, but generally are genetically dominant and have a low degree of polymorphism.