Epidemic history of hepatitis C virus infection in two remote communities in Nigeria, West Africa
Epidemic history of hepatitis C virus infection in two remote communities in Nigeria, West Africa
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DOI：
10.1099/vir.0.042184-0
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发表时间：
2012-07-01
影响因子：
3.8
通讯作者：
Khudyakov, Yury E.
中科院分区：
文献类型：
--
作者：
Forbi, Joseph C.;Purdy, Michael A.;Khudyakov, Yury E.
我们调查了尼日利亚中北部两个半隔离社区的原住民中HCV感染的分子流行病学和群体动态。尽管地处偏远和隔离，但仍有约15%的人口具有丙型肝炎病毒（HCV）感染的血清学或分子标志物。对从60名HCV感染居民中获得的NS 5 b序列进行系统发育分析显示，HCV变种属于基因型1（n=51; 85%）和基因型2（n=9; 15%）。所有序列都是独特的，并与来自其他西非国家感染者的HCV序列在系统发育树中混合。采用HCV高变区1的高通量454焦磷酸测序和经验阈值误差校正算法来评估来自社区居民的基因型1（n=43）和基因型2（n=6）的HCV毒株的宿主内异质性。分析显示，居民中罕见的HCV宿主内变异的混合。在所有已知的亲属群体中识别出基因上接近的HCV变异体表明社区中常见的家族内HCV传播。应用贝叶斯结合分析的NS 5 b序列，最近的共同祖先基因型1和2变异估计已经分别存在675年和286年前。贝叶斯天际线图表明，在尼日利亚社区中鉴定的两种基因型的HCV谱系经历了200-300年的流行增长，直到世纪中期。这些数据表明，在世纪，在尼日利亚过去三个世纪丙型肝炎流行的动态演变历史背景下，大量HCV变异体被引入社区。
We investigated the molecular epidemiology and population dynamics of HCV infection among indigenes of two semi-isolated communities in North-Central Nigeria. Despite remoteness and isolation, similar to 15% of the population had serological or molecular markers of hepatitis C virus (HCV) infection. Phylogenetic analysis of the NS5b sequences obtained from 60 HCV-infected residents showed that HCV variants belonged to genotype 1 (n=51; 85%) and genotype 2 (n=9; 15%). All sequences were unique and intermixed in the phylogenetic tree with HCV sequences from people infected from other West African countries. The high-throughput 454 pyrosequencing of the HCV hypervariable region 1 and an empirical threshold error correction algorithm were used to evaluate intra-host heterogeneity of HCV strains of genotype 1 (n=43) and genotype 2 (n=6) from residents of the communities. Analysis revealed a rare detectable intermixing of HCV intrahost variants among residents. Identification of genetically close HCV variants among all known groups of relatives suggests a common intra-familial HCV transmission in the communities. Applying Bayesian coalescent analysis to the NS5b sequences, the most recent common ancestors for genotype 1 and 2 variants were estimated to have existed 675 and 286 years ago, respectively. Bayesian skyline plots suggest that HCV lineages of both genotypes identified in the Nigerian communities experienced epidemic growth for 200-300 years until the mid-20th century. The data suggest a massive introduction of numerous HCV variants to the communities during the 20th century in the background of a dynamic evolutionary history of the hepatitis C epidemic in Nigeria over the past three centuries.