Infection-related declines in chill coma recovery and negative geotaxis in Drosophila melanogaster.
Infection-related declines in chill coma recovery and negative geotaxis in Drosophila melanogaster.
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DOI：
10.1371/journal.pone.0041907
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Schneider DS
中科院分区：
文献类型：
--
作者：
Linderman JA;Chambers MC;Gupta AS;Schneider DS
对果蝇感染的研究提供了对宿主抵抗和病原体耐受机制的深入了解。然而，对这些过程所涉及途径的研究受到了限制，因为可用于衡量整个感染过程中疾病和健康状况的指标相对较少。在这里，我们报告了两种不同行为测定中果蝇表现与感染相关的下降的测量结果。在感染单核细胞增生李斯特菌或肺炎链球菌期间，黑腹果蝇从寒冷引起的昏迷中恢复较慢。单核细胞增多性李斯特菌感染也会影响果蝇在负趋地性测定中的表现，表明它们的攀爬率下降，这是它们受惊后先天逃避反应的一部分。除了提供评估健康状况的新措施外，这些检测还表明感染过程中可能发生的病理后果和代谢变化。
Studies of infection in Drosophila melanogaster provide insight into both mechanisms of host resistance and tolerance of pathogens. However, research into the pathways involved in these processes has been limited by the relatively few metrics that can be used to measure sickness and health throughout the course of infection. Here we report measurements of infection-related declines in flies' performance on two different behavioral assays. D. melanogaster are slower to recover from a chill-induced coma during infection with either Listeria monocytogenes or Streptococcus pneumoniae. L. monocytogenes infection also impacts flies' performance during a negative geotaxis assay, revealing a decline in their rate of climbing as part of their innate escape response after startle. In addition to providing new measures for assessing health, these assays also suggest pathological consequences of and metabolic shifts that may occur over the course of an infection.