New Perspectives on the Structure and Function of Ubiquitin
New Perspectives on the Structure and Function of Ubiquitin
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DOI：
10.1038/nbt0390-209
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发表时间：
1990-03
期刊：
影响因子：
--
通讯作者：
B. Monia;D. Ecker;S. Crooke
中科院分区：
文献类型：
--
作者：
B. Monia;D. Ecker;S. Crooke
泛素被认为在广泛的基本细胞功能中发挥关键作用，从基因表达的差异调节到蛋白质的降解。最近对天然和人工合成泛素基因融合的研究导致了关于细胞中泛素系统的新功能、蛋白质降解处理机制以及泛素融合技术在细菌和酵母中增强异源基因产物表达的重要发现。此外，涉及定点突变和二维核磁共振的研究已经证明泛素是蛋白质工程的一个很好的模型，并导致了关于其在ATP依赖的蛋白质降解中的作用机制的重要发现。最后，最近泛素羧基延伸蛋白作为核糖体蛋白的发现和鉴定开辟了泛素相关研究的新领域，并有助于解释作为泛素融合的异源基因产物增加表达的机制。
Ubiquitin has been suggested to play a key role in a wide variety of essential cellular functions ranging from differential regulation of gene expression to protein degradation. Recent studies on natural and synthetic ubiquitin gene fusions have led to important discoveries concerning novel functions for the ubiquitin system in cells, mechanisms of proteolytic processing, and the development of a ubiquitin fusion technology for augmenting the expression of heterologous gene products in bacteria and yeast. Furthermore, studies involving site-directed mutagenesis and two-dimensional NMR have proven ubiquitin to be an excellent model for protein engineering and have led to important discoveries concerning its mechanism of action in ATP-dependent proteolysis. Finally, the recent identification and characterization of ubiquitin carboxyl extension proteins as ribosomal proteins has opened up an even newer area of ubiquitin-related research and has helped to explain the mechanisms involved in increasing the expression of heterologous gene products made as ubiquitin fusions.