Taxonomy, diversity and origins of symbiotic endophytes of Achnatherum sibiricum in the Inner Mongolia Steppe of China.
Taxonomy, diversity and origins of symbiotic endophytes of Achnatherum sibiricum in the Inner Mongolia Steppe of China.
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DOI：
10.1111/j.1574-6968.2009.01789.x
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发表时间：
2009-12
影响因子：
2.1
通讯作者：
Xin Zhang;A. Ren;Yu-Kun Wei;Feng Lin;Chuan Li;Zhi-jian Liu;Yu-bao Gao
中科院分区：
文献类型：
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作者：
Xin Zhang;A. Ren;Yu-Kun Wei;Feng Lin;Chuan Li;Zhi-jian Liu;Yu-bao Gao
我们采样了五个Achnatherum sibiricum种群，并记录了内生菌的分布和丰度。共获得438株真菌分离株。根据菌落形态、产孢细胞产生量、分生孢子大小及在马铃薯葡萄糖琼脂培养基上的生长速度和形态特征，将它们分为11个形态型。两个tub2和actG序列确定只有一个单一的产品，在所有代表性的分离株从三个主要的形态型。所有序列都属于同一个主分支，包括Neotyphodium gansuense和Neotyphodium gansuense var. inebrians和Neotyphodium guerinii。根据形态学和系统发育分析，形态型I的分离物被描述为一个新的Neotyphodium种，Neotyphodium sibiricum sp. nov.。甘肃的。tub2和actG序列均表明N. sibiricum和N. guerinii可能来自一个共同的Epichloë祖先。
We sampled five Achnatherum sibiricum populations and documented the distribution and abundance of endophytes. In total, 438 fungal isolates were obtained. They were classified into 11 morphotypes based on growth rate and morphological characters including colonial morphology, the production of conidiogenous cells, conidial size and dimension on potato dextrose agar medium. Both tub2 and actG sequences identified only a single product in all representative isolates from three dominant morphotypes. All sequences fell in the same main clade, including Neotyphodium gansuense and Neotyphodium gansuense var. inebrians from another Achnatherum species in North China, Achnatherum inebrians, and Neotyphodium guerinii from European grasses Melica ciliate and Melica transsilvanica. Based on both morphological and phylogenetic analyses, isolates in morphotype I were described as representing a novel Neotyphodium species, Neotyphodium sibiricum sp. nov. Isolates in both morphotype II and III were identified as representing N. gansuense. Both tub2 and actG sequences suggested that N. sibiricum and N. guerinii were probably derived from a common Epichloë ancestor.