GENETIC-TRANSFORMATION OF VARIOUS SPECIES OF ENTEROCOCCUS BY ELECTROPORATION
GENETIC-TRANSFORMATION OF VARIOUS SPECIES OF ENTEROCOCCUS BY ELECTROPORATION
复制标题
DOI：
10.1016/0378-1097(91)90106-k
复制
发表时间：
1991-04-15
影响因子：
2.1
通讯作者：
WIRTH, R
中科院分区：
文献类型：
--
作者：
FRIESENEGGER, A;FIEDLER, S;WIRTH, R
利用未经处理的(即非原生质体)细胞和电穿孔技术建立了粪肠球菌的转化系统。优化后的转化子转化效率高达4×10(6)个转化子/mU-g。所有被测试的粪肠球菌菌株都可以用这种方法转化，尽管转化效率不同。利用针对粪肠球菌优化的方法，我们成功地转化了粪肠球菌、平肠球菌、恶臭肠球菌和蒙氏肠球菌。
A transformation system for Enterococcus faecalis was developed which uses untreated (i.e. non-protoplasted) cells and the electroporation technique. The optimized protocol resulted in transformation efficiencies of up to 4 x 10(6) transformants per mu-g of plasmid DNA. All strains of E. faecalis tested could be transformed by this method, albeit with differing transformation efficiencies. Using the protocol optimized for E. faecalis we successfully transformed Enterococcus faecium, E. hirae, E. malodoratus and E. mundtii.