INOCULUM-DEPENDENT DIVISION LAG OF BACILLUS CULTURES AND ITS RELATION TO AN ENDOGENOUS FACTOR(S) (SCHIZOKINEN)
INOCULUM-DEPENDENT DIVISION LAG OF BACILLUS CULTURES AND ITS RELATION TO AN ENDOGENOUS FACTOR(S) (SCHIZOKINEN)
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DOI：
10.1128/jb.91.3.1070-1079.1966
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发表时间：
1966-01-01
影响因子：
3.2
通讯作者：
JONES, RJ
中科院分区：
文献类型：
--
作者：
LANKFORD, CE;WALKER, JR;JONES, RJ
当巨大芽孢杆菌的细胞，生长在脑心浸液琼脂，接种到化学成分确定的培养基，它们表现出分裂滞后，这是接种量的反函数。从相同的培养基中添加100%的培养物消除了滞后的接种物依赖性成分，但不是恒定持续时间的接种物独立的残留滞后。B的培养滤液。枯草尼日尔不仅消除了依赖接种的滞后现象，而且还需要维持指数分裂。剂量反应和“生长时间”的生物测定，开发来测量滞后减少活性的BMPs，证明积累的活性过滤因子的“临界”浓度之前，分裂开始。向培养物中添加该浓度消除了接种物依赖性滞后。积累的因素暂时停止分裂开始时，但排泄恢复后，在指数增长。一个滞后减少，细胞相关因子的积累遵循类似的过程。B培养液的色谱和生物自显影分析。megaterium表明一种单一的物质是主要负责他们的活性。某些因子对不同因子的相互作用以及其他因子对不同数量的相似因子的相互作用的剂量反应试验结果。有人建议，这种内源性因子的合成和积累，以人口依赖的浓度作为必要的启动和维持分裂被指定为“Escherokinens。".
When cells of Bacillus megaterium, grown on Brain Heart Infusion Agar, were inoculated into a chemically defined medium, they exhibited a division lag which was an inverse function of inoculum size. The addition of filtrates of cultures from the same medium eliminated the inoculum-dependent component of lag, but not an inoculum-independent residual lag of constant duration. Culture filtrate of B. subtilis var. niger not only eliminated its inoculum-dependent lag but also was required to sustain exponential division. Dose-response and "growth time" bioassays, developed to measure lag-reducing activity of filtrates, demonstrated accumulation of active filtrate factor to a "critical" concentration prior to division initiation. Addition of this concentration to cultures eliminated the inoculum-dependent lag. Accumulation of the factor ceased temporarily at onset of division, but excretion was resumed later during exponential growth. Accumulation of a lag-reducing, cell-associated factor followed a similar course. Chromatographic and bioautographic analyses of culture filtrates of B. megaterium indicated that a single substance was primarily responsible for their acitivty. Results of dose-response tests for reciprocal activities of filtrates of different factors by some, and of different quantities of similar factors by others. It is proposed that such endogenous factors which are synthesized and accumulate to a population-dependent concentration as a requisite to initiation and maintenance of division be designated as "schizokinens.".