Bisphenol A induced oxidative stress mediated genotoxicity in Drosophila melanogaster
Bisphenol A induced oxidative stress mediated genotoxicity in Drosophila melanogaster
复制标题
DOI：
10.1016/j.jhazmat.2018.07.050
复制
发表时间：
2019-05-15
影响因子：
13.6
通讯作者：
Puttaswamygowda, Gurushankara Hunasanahally
中科院分区：
文献类型：
--
作者：
Anet, Antony;Olakkaran, Shilpa;Puttaswamygowda, Gurushankara Hunasanahally
本研究调查了双酚 A (BPA) 诱导的氧化应激介导体内黑腹果蝇模型的遗传毒性。计算得出的 BPA LC50 为 12.35 μg/mL。黑腹果蝇菌株从胚胎阶段（卵）起就在0.1、1.0、2.5和5.0μg/mL BPA处理的食品培养基中饲养；分析氧化应激和遗传毒性参数。食物摄入量分析证实，BPA 不是果蝇幼虫的拒食剂，并且果蝇幼虫食用含有 BPA 的食物。与对照组相比，BPA 处理组的活性氧 (ROS) 和脂质过氧化 (LPO) 增加，超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、谷胱甘肽 (GSH) 和谷胱甘肽-s-转移酶 (GST) 抗氧化活性降低。在标记杂合子（MH；mwh/flr3）和平衡杂合子（BH；mwh/TM3）基因型果蝇的标准（ST）和高生物活性（HB）杂交中观察到阳性单点/翅频率，表明BPA具有诱变性而非重组性。 BPA 处理后彗星测定中尾部长度和尾部 DNA 百分比显着增加，表明 BPA 具有诱导遗传毒性的潜力。目前的研究表明，BPA 暴露会诱导氧化应激，这可能是诱导遗传毒性的可能机制之一。
This study investigates Bisphenol A (BPA) induced oxidative stress that mediates the genotoxicity in in vivo model Drosophila melanogaster. The calculated LC50 for BPA was 12.35 mu g/mL. The strains of D. melanogaster were reared in 0.1, 1.0, 2.5 and 5.0 mu g/mL BPA treated food media from the embryonic stage (egg); oxidative stress and genotoxicity parameters were analyzed. Food intake analysis confirmed that BPA is not an anti feedant for Drosophila larvae and it consumed BPA containing food. Increased reactive oxygen species (ROS) and lipid peroxidation (LPO) and depletion of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione-s-transferase (GST) antioxidant activities were observed in BPA treated groups compared to control. Positive single spots/wing frequencies were observed in standard (ST) and high bioactivation (HB) crosses of marker heterozygous (MH; mwh/flr3) and balancer heterozygous (BH; mwh/TM3) genotype flies indicating BPA is mutagenic and not recombinogenic. A significant increase in tail length and % tail DNA in Comet assay after BPA treatment reveals that BPA has a potential to induce the genotoxicity. Present study suggests that BPA exposure induces oxidative stress, which could be one of the possible mechanisms for induction of genotoxicity.