TUBULIN EVOLUTION - CILIATE-SPECIFIC EPITOPES ARE CONSERVED IN THE CILIARY TUBULIN OF METAZOA
TUBULIN EVOLUTION - CILIATE-SPECIFIC EPITOPES ARE CONSERVED IN THE CILIARY TUBULIN OF METAZOA
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DOI：
10.1007/bf02099751
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发表时间：
1985-01-01
影响因子：
3.9
通讯作者：
BEISSON, J
中科院分区：
文献类型：
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作者：
ADOUTTE, A;CLAISSE, M;BEISSON, J
尽管纤毛虫的微管蛋白总体上具有进化保守性，但它们表现出电泳和结构的特殊性。我们在此表明，针对草履虫和四膜虫纤毛微管蛋白产生的抗体无法识别所有测试的后生动物的细胞质微管蛋白。纤毛虫微管蛋白肽图的免疫印迹显示，这些抗体与一种或极少数纤毛虫特异性表位发生反应，而针对脊椎动物微管蛋白的多克隆抗体相当于自身抗体，可识别纤毛虫和脊椎动物微管蛋白中的多个表位。此外，我们发现抗纤毛虫抗体可以识别从海胆到哺乳动物（人类除外）的后生动物的纤毛和鞭毛微管蛋白。结果支持这样的结论：尽管后生动物中微管蛋白基因的复制和特化可能导致了不同类型的微管蛋白，但轴丝蛋白基因仍然高度保守。
In spite of their overall evolutionary conservation, the tubulins of ciliates display electrophoretic and structural particularities. We show here that antibodies raised against Paramecium and Tetrahymena ciliary tubulins fail to recognize the cytoplasmic tubulins of all the metazoans tested. Immunoblotting of peptide maps of ciliate tubulins reveals that these antibodies react with one or very few ciliate-specific epitopes, in contrast to polyclonal antibodies against vertebrate tubulins, which are equivalent to autoantibodies and recognize several epitopes in both ciliate and vertebrate tubulins. Furthermore, we show that the anti-ciliate antibodies recognize ciliary and flagellar tubulins of metazoans ranging from sea urchin to mammals (with the exception of humans). The results support the conclusion that although duplication and specialization of tubulin genes in metazoans may have led to distinct types of tubulins, the axonemal one has remained highly conserved.