Differentiation of lepidoptera scale cells from epidermal stem cells followed by ecdysone-regulated DNA duplication and scale secreting
Differentiation of lepidoptera scale cells from epidermal stem cells followed by ecdysone-regulated DNA duplication and scale secreting
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鳞翅目鳞片细胞从表皮干细胞分化，然后进行蜕皮激素调节的 DNA 复制和鳞片分泌
DOI：
10.1080/15384101.2017.1376148
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发表时间：
2017-09
期刊：
影响因子：
4.3
通讯作者：
Erjun Ling
中科院分区：
文献类型：
--
作者：
Shenglei Yuan;Wuren Huang;Lei Geng;Brenda T. Beerntsen;Hongsheng Song;Erjun Ling
摘要被膜是保护昆虫免受物理伤害和病原体感染的第一道防线。在鳞翅目昆虫中，它们在变态期间经历明显的形态变化，例如鳞片的形成。然而，我们对这一阶段的珠被发育和鳞片形成知之甚少。在这里，我们使用的家蚕，家蚕，作为一个模型，并表明，干细胞在珠被的每个部分，但不是intersegmental膜，分为两个规模的前体细胞在纺纱阶段。在幼年脓疱中，鳞片前体细胞再次分裂。其中一个子细胞经过几轮DNA复制后成为成熟的鳞片分泌细胞，而另一个子细胞随后经历凋亡。这种鳞片前体细胞分裂对鳞片分泌细胞的发育和分化至关重要，因为用细胞分裂抑制剂紫杉醇处理后可以阻断鳞片的产生。随后，鳞片分泌细胞的生长受到20-羟基蜕皮激素而不是保幼激素的控制，因为注射20-羟基蜕皮激素抑制了鳞片的形成。进一步的研究表明，20-羟基蜕皮激素注射液抑制了鳞片分泌细胞中的DNA复制，而BR-C Z2下调了鳞片形成基因ASH 1的表达。因此，本研究表明，家蚕的鳞片细胞在化蛹前通过干细胞分裂发育，然后在进入蛹期后不久，另一波细胞分裂将这些细胞分化为鳞片分泌细胞。此外，DNA复制和规模生产的规模分泌细胞被发现的20-羟基蜕皮激素的调节。
ABSTRACT Integuments are the first line to protect insects from physical damage and pathogenic infection. In lepidopteran insects, they undergo distinct morphology changes such as scale formation during metamorphosis. However, we know little about integument development and scale formation during this stage. Here, we use the silkworm, Bombyx mori, as a model and show that stem cells in the integument of each segment, but not intersegmental membrane, divide into two scale precursor cells during the spinning stage. In young pupae, the scale precursor cell divides again. One of the daughter cells becomes a mature scale-secreting cell that undergoes several rounds of DNA duplication and the other daughter cell undergoes apoptosis later on. This scale precursor cell division is crucial to the development and differentiation of scale-secreting cells because scale production can be blocked after treatment with the cell division inhibitor paclitaxel. Subsequently, the growth of scale-secreting cells is under the control of 20-hydroxyecdysone but not juvenile hormone since injection of 20-hydroxyecdysone inhibited scale formation. Further work demonstrated that 20-hydroxyecdysone injection inhibits DNA duplication in scale-secreting cells while the expression of scale-forming gene ASH1 was down-regulated by BR-C Z2. Therefore, this research demonstrates that the scale cells of the silkworm develops through stem cell division prior to pupation and then another wave of cell division differentiates these cells into scale secreting cells soon after entrance into the pupal stage. Additionally, DNA duplication and scale production in the scale-secreting cells were found to be under the regulation of 20-hydroxyecdysone.
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