Ptf1a, Lbx1 and Pax2 coordinate glycinergic and peptidergic transmitter phenotypes in dorsal spinal inhibitory neurons
Ptf1a, Lbx1 and Pax2 coordinate glycinergic and peptidergic transmitter phenotypes in dorsal spinal inhibitory neurons
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Ptf1a、Lbx1 和 Pax2 协调背脊髓抑制神经元中的甘氨酸能和肽能递质表型
DOI：
10.1016/j.ydbio.2008.06.031
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发表时间：
2008-10-15
影响因子：
2.7
通讯作者：
Cheng, Leping
中科院分区：
文献类型：
--
作者：
Huang, Menggui;Huang, Tianwen;Cheng, Leping
背角的抑制性神经元合成多种神经递质，包括GABA、甘氨酸和一组多肽。在这里，我们展示了三种转录因子，Prf1a， Pax2和Lbx1，它们已被报道促进gaba能细胞的命运，也指定甘氨酸能和肽能递质表型。首先，Prf1a似乎是一个主调节因子，正如Prf1a需要表达甘氨酸能标记物GlyT2和一组肽，包括神经肽Y (NPY)，痛觉肽/孤儿蛋白FQ（N/OFQ）所表明的那样。生长抑素（SOM）、脑啡肽（ENK）、促啡肽（DYN）和丙氨酸（GAL）。其次，Pax2是Prf1a的下游靶点，并控制递质表型的亚群，包括GlyT2， NPY, N/OFQ， DYN和GAL的表达，但对于SOM of ENK的表达是必不可少的。第三，对于Lbx1，由于后期神经元细胞的损失，我们的分析集中在胚胎早期，我们发现Lbx1是GlyT2， NPY， N/OFQ的表达所必需的，并且部分负责SOM的表达。因此，我们的研究表明，脊背抑制性神经元中各种神经递质具有协调和分层的特征。(C) 2008爱思唯尔公司版权所有。
inhibitory neurons in the dorsal horn synthesize a variety of neurotransmitters, including GABA, glycine and a set of peptides. Here we show that three transcription factors, Prf1a, Pax2, and Lbx1, which have been reported to promote a GABAergic cell fate, also specify glycinergic and peptidergic transmitter phenotypes. First, Prf1a appears to be a master regulator, as indicated by a requirement of Prf1a for the expression of glycinergic marker GlyT2 and a set of peptides, including neuropeptide Y (NPY), nociceptin/orphanin FQ(N/OFQ). somatostatin (SOM), enkephalin (ENK), dynorphin (DYN) and galanin (GAL). Second, Pax2 is a downstream target of Prf1a and controls subsets of transmitter phenotypes, including the expression of GlyT2, NPY, N/OFQ, DYN, and GAL, but is dispensable for SOM OF ENK expression. Third, for Lbx1, due to neuronal cell loss at late stages, our analyses focused on early embryonic stages, and we found that Lbx1 is required for the expression of GlyT2, NPY, N/OFQ and is partially responsible for SOM expression. Our Studies therefore Suggest a coordinated and hierarchical specification of a variety of neurotransmitters in dorsal spinal inhibitory neurons. (C) 2008 Elsevier Inc. All rights reserved.