The Pur10 protein encoded in the gene cluster for puromycin biosynthesis of Streptomyces alboniger is an NAD-dependent ATP dehydrogenase
The Pur10 protein encoded in the gene cluster for puromycin biosynthesis of Streptomyces alboniger is an NAD-dependent ATP dehydrogenase
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DOI：
10.1016/s0014-5793(98)01228-9
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发表时间：
1998-10-23
期刊：
影响因子：
3.5
通讯作者：
Jiménez, A
中科院分区：
文献类型：
--
作者：
Rubio, MA;Espinosa, JC;Jiménez, A
白黑链霉菌嘌呤霉素簇的pur 10基因是合成抗生素所必需的。在大肠杆菌中获得的高度纯化的Pur 10蛋白作为与组氨酸尾部融合的重组蛋白，具有NAD依赖性ATP脱氢酶活性，ATP的Km和Vmax值分别为0.49 mM和14.5 nmol/min，NAD的Km和Vmax值分别为0.53 mM和15.2 nmol/min。反应的ATP衍生产物明显分解，产生三磷酸化化合物和腺嘌呤衍生物。这些和以前的结果表明，Pur 10进行嘌呤霉素生物合成途径的第一步，即ATP转化为3 '-酮-3'-脱氧ATP。(C)1998年欧洲生物化学学会联合会。
The pur10 gene of the puromycin (pur) cluster of Streptomyces alboniger is essential for the biosynthesis; of thin antibiotic. Highly purified Pur10 protein, obtained in Escherichia coli as a recombinant protein fused to a histidine tail, had an NAD-dependent ATP dehydrogenase activity, The K-m and V-max values for ATP were 0.49 mM and 14.5 nmol/min and for NAD 0.53 mM and 15.2 nmol/min, respectively. The ATP-derived product of the reaction apparently decomposed producing a triphosphorylated compound plus an adenine derivative. These and previous results suggested that Pur10 carries out the first step of the puromycin biosynthetic pathway, namely, conversion of ATP into 3'-keto-3'-deoxyATP. (C) 1998 Federation of European Biochemical Societies.