Molecular Cloning and Characterization of BcMF22, a Novel Gene Related to Pollen Development and Fertilization in Brassica campestris ssp. chinensis
Molecular Cloning and Characterization of BcMF22, a Novel Gene Related to Pollen Development and Fertilization in Brassica campestris ssp. chinensis
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DOI：
10.1007/s11105-011-0393-3
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发表时间：
2012-01
影响因子：
2.1
通讯作者：
Yifei Li;Lin Qiu;Li Huang;Jiashu Cao
中科院分区：
文献类型：
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作者：
Yifei Li;Lin Qiu;Li Huang;Jiashu Cao
从大白菜（B.坎培斯特里湖中国刺蕨（Ssp.chinensisMakino），syn.B. rapassp.chinensis）。BcMF 22的cDNA克隆长度为1，870 bp，含有1，260 bp的开放阅读框（OFF），而基因组序列不含内含子。序列预测表明BcMF22可能编码一个甲基转移酶。时空表达分析表明BcMF22在花粉中优先表达。BcMF22的转录本首先在花粉母细胞中检测到，并继续存在于成熟花粉中。此外，转录检测授粉雌蕊，授粉后约4小时（4HAP）。这些结果表明，BcMF22可能是一个花粉偏好基因，与授粉密切相关。
TheBcMF22(Brassica campestris Male Fertility 22) gene was isolated from Chinese cabbage-pak-choi (B. campestrisL. ssp.chinensisMakino, syn.B. rapassp.chinensis). The cDNA clone ofBcMF22was 1,870 bp in length and contained an open reading frame (OFF) of 1,260 bp, while the genomic sequence contained no introns. Sequence prediction indicated thatBcMF22might encode a methytransferase. Spatial and temporal expression analyses indicatedBcMF22was preferentially expressed in pollen. Transcripts ofBcMF22were first detected in pollen mother cells and continued to be present in mature pollen. In addition, transcripts were detected in pollinated pistils, approximately 4 h after pollination (4HAP). These results indicated thatBcMF22might be a pollen-preferential gene and was closely involved in pollination.