Cellular fine structures and histochemical reactions in the tissue of a cypress twig preserved in Baltic amber
Cellular fine structures and histochemical reactions in the tissue of a cypress twig preserved in Baltic amber
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DOI：
10.1098/rspb.2004.2939
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发表时间：
2005-01-22
影响因子：
4.7
通讯作者：
Tischendorf, G
中科院分区：
文献类型：
--
作者：
Koller, B;Schmitt, JM;Tischendorf, G
一个柏树植物的树枝保存约。通过光学和电子显微镜分析了波罗的海琥珀中的4500万年。整个植物的横截面显示整个茎和叶的几乎完整的组织，据我们所知，这是迄今为止报道的琥珀内含物中最古老和保存最完好的组织。该制备基于内部嵌入的新技术，其中内含物内的中空空间填充有合成树脂，其在切片过程中稳定细胞结构。应用于切片的细胞学染色剂与细胞壁和细胞核反应。观察到叶片角质层和树脂道发出强烈的绿色自发荧光。透射电子显微镜照片显示高度保存的细胞壁，膜和细胞器的精细结构。结果进行了比较，与分类学相关的最近Glyptostrobus和刺柏属植物。
A twig of a cypress plant preserved for ca. 45 Myr in Baltic amber was analysed by light and electron microscopy. Cross-sections of the whole plant showed an almost intact tissue of the entire stem and leaves, revealing, to our knowledge, the oldest and most highly preserved tissue from an amber inclusion reported so far. The preparations are based on a new technique of internal imbedding, whereby the hollow spaces within the inclusion are filled with synthetic resin which stabilizes the cellular structures during the sectioning procedure. Cytological stains applied to the sections reacted with cell walls and nuclei. A strong green auto-fluorescence of the cuticle and the resin canals in the leaves was observed. Transmission electron micrographs revealed highly preserved fine structures of cell walls, membranes and organelles. The results were compared with taxonomically related recent Glyptostrobus and Juniperus plants.