Assessment of intervertebral disc degeneration based on quantitative magnetic resonance imaging analysis: an in vivo study.
Assessment of intervertebral disc degeneration based on quantitative magnetic resonance imaging analysis: an in vivo study.
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DOI：
10.1097/brs.0000000000000194
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发表时间：
2014-03-15
期刊：
影响因子：
3
通讯作者：
Härtl R
中科院分区：
文献类型：
--
作者：
Grunert P;Hudson KD;Macielak MR;Aronowitz E;Borde BH;Alimi M;Njoku I;Ballon D;Tsiouris AJ;Bonassar LJ;Härtl R
动物实验研究评价一种新的定量成像技术用于评估椎间盘退变。T2弛豫时间（T2-RT）测量已被用于定量评估椎间盘退变。T2值与椎间盘组织的含水量相关，从而允许间接测量髓核（NP）水合作用。我们开发了一种算法，根据T2-RT值减去不代表NP组织的MRI体素。过滤后的NP体素用于测量核大小的数量和核水合的平均T2-RT。这种技术被应用于24个大鼠尾椎间盘（IVD），这已被用18号针穿刺根据不同的技术，以诱导不同程度的退变。使用NP体素计数和平均T2-RT作为参数来评估穿刺后1个月和3个月的退变过程。根据X线椎间盘高度测量和基于Pfirrmann分级系统的定性MRI研究评价NP体素计数。收集尾部进行组织学检查，以将NP体素计数与组织学椎间盘退变等级和NP横截面积测量值相关联。NP体素计数测量显示与定性MRI分析（R2=0.79，p<0.0001）、组织学变性等级（R2=0.902，p<0.0001）和组织学NP横截面积测量（R2=0.887，p<0.0001）的强相关性。与NP体素计数相反，每个穿刺组的平均T2-RT在第1个月和第3个月之间保持恒定。在任一时间点，穿刺组的平均T2-RT与健康IVD组的平均T2-RT（第1个月63.55 ms ± 5.88 ms和62.61 ms ± 5.02 ms）无统计学显著差异。NP体素计数被证明是在针刺模型中定量评估椎间盘退变的有效参数。平均NP T2-RT在针刺诱导的退化IVD中没有显著变化。IVD可以根据其先天T2-RT分为不同的组织成分。
Animal experimental study To evaluate a novel quantitative imaging technique for assessing disc degeneration. T2-relaxation time (T2-RT) measurements have been used to quantitatively assess disc degeneration. T2 values correlate with the water content of inter vertebral disc tissue and thereby allow for the indirect measurement of nucleus pulposus (NP) hydration. We developed an algorithm to subtract out MRI voxels not representing NP tissue based on T2-RT values. Filtered NP voxels were used to measure nuclear size by their amount and nuclear hydration by their mean T2-RT. This technique was applied to 24 rat-tail intervertebral discs’ (IVDs), which had been punctured with an 18-gauge needle according to different techniques to induce varying degrees of degeneration. NP voxel count and average T2-RT were used as parameters to assess the degeneration process at 1 and 3 months post puncture. NP voxel counts were evaluated against X-ray disc height measurements and qualitative MRI studies based on the Pfirrmann grading system. Tails were collected for histology to correlate NP voxel counts to histological disc degeneration grades and to NP cross-sectional area measurements. NP voxel count measurements showed strong correlations to qualitative MRI analyses (R2=0.79, p<0.0001), histological degeneration grades (R2=0.902, p<0.0001) and histological NP cross-sectional area measurements (R2=0.887, p<0.0001). In contrast to NP voxel counts, the mean T2-RT for each punctured group remained constant between months 1 and 3. The mean T2-RTs for the punctured groups did not show a statistically significant difference from those of healthy IVDs (63.55ms ±5.88ms month 1 and 62.61ms ±5.02ms) at either time point. The NP voxel count proved to be a valid parameter to quantitatively assess disc degeneration in a needle puncture model. The mean NP T2-RT does not change significantly in needle-puncture induced degenerated IVDs. IVDs can be segmented into different tissue components according to their innate T2-RT.