DEVELOPMENT AND REMODELING OF CEREBRAL BLOOD-VESSELS AND THEIR FLOW IN POSTNATAL MICE OBSERVED WITH INVIVO VIDEOMICROSCOPY
DEVELOPMENT AND REMODELING OF CEREBRAL BLOOD-VESSELS AND THEIR FLOW IN POSTNATAL MICE OBSERVED WITH INVIVO VIDEOMICROSCOPY
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DOI：
10.1038/jcbfm.1992.130
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发表时间：
1992-11-01
影响因子：
6.3
通讯作者：
WOOLSEY, TA
中科院分区：
文献类型：
--
作者：
WANG, DB;BLOCHER, NC;WOOLSEY, TA
从出生（P0）到成年，评估小鼠躯体感觉（桶）皮层中血管的变化。应用视频显微镜通过闭合的颅窗直接观察表面血管解剖和血流，并应用血管内荧光示踪剂。组织学用于确定内部毛细血管密度。在出生时，小动脉有许多相互重叠，软脑膜毛细血管形成密集的表面丛，软脑膜小静脉和静脉相对较小且不规则。接下来2周的形态学变化包括：（a）小动脉闭塞减少，（B）小静脉形成和生长，（c）所有类型血管节段的直径更均匀，（d）实质内毛细血管长度密度（L（v））增加，（e）浅表毛细血管密度和直径降低。一个简单的形态学测试表明，在新生儿和成人的小动脉分支点的壁剪切率平均匹配。评价了单支血管中的流动特性。在直径相似的小动脉中，（a）V（max），（B）峰值与剪切率，（c）峰值流量在所有年龄段都相似;（d）新生儿中偶尔发生动静脉（AV）分流时流速非常高;（e）小动脉闭塞时流速缓慢且可变。尽管在所有类型的血管中流动是不均匀的，但是新生小鼠和成年小鼠中相同尺寸的小动脉中的平均峰值速度和计算的壁剪切速率的显著相似性表明，血流在发育期间部分地调节血管的重塑（Rovainen等人，1992年）。啮齿动物桶皮质经历主要的神经元和血管的发展，功能分化，并在出生后的第一周重塑。它为测试血管如何生长和适应大脑神经模块的局部代谢需求提供了特殊的机会。
Changes of blood vessels in the mouse somatosensory (barrel) cortex were assessed from birth (P0) to adulthood. Sur-face vessel anatomy and flow were observed directly with videomicroscopy through closed cranial windows and with intravascular fluorescent tracers. Histology was used to determine the internal capillary density. At birth, arterioles had numerous anastomoses with each other, pial capillaries formed a dense surface plexus, and pial venules and veins were relatively small and irregular. Morphological changes over the next 2 weeks included (a) fewer arteriolar anastomoses, (b) formation and growth of venules, (c) more uniform diameters of all types of vascular segments, (d) increase in intraparenchymal capillary length density (L(v)), and (e) decreases in superficial capillary density and diameters. A simple morphological test showed that wall shear rates at arteriolar branch points were matched on average in neonates and adults. Flow characteristics in single vessels were evaluated. In arterioles of like diameters, (a) V(max), (b) peak with shear rates, and (c) peak flows were similar at all ages; (d) velocity was very high in occasional arteriovenous (AV) shunts in newborns; and (e) flow in arteriolar anastomoses was slow and variable. Although flow was heterogeneous in all types of vessel, the marked similarities in newborn and adult mice of average peak velocities and calculated wall shear rates in arterioles of the same size suggest that blood flow regulates in part the remodeling of blood vessels during development (Rovainen et al., 1992). The rodent barrel cortex undergoes major neuronal and vascular development, functional differentiation, and remodeling during the first weeks after birth. It provides special opportunities for testing how blood vessels grow and adapt to supply the local metabolic requirements of neural modules in the brain.