Applicability of enzymatic extracts obtained by solid state fermentation on grape pomace and orange peels mixtures in must clarification
Applicability of enzymatic extracts obtained by solid state fermentation on grape pomace and orange peels mixtures in must clarification
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DOI：
10.1016/j.lwt.2010.12.006
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发表时间：
2011-05-01
影响因子：
6
通讯作者：
Blandino, Ana
中科院分区：
文献类型：
--
作者：
Diaz, Ana Belen;Bolivar, Jorge;Blandino, Ana
葡萄酒生产过程中产生的葡萄渣是一个环境问题。另一方面，酶提取物在葡萄酒酿造中是非常有价值的产品。在这项工作中，我们评估的适用性必须澄清的酶提取物产生的固态发酵的葡萄渣和橙子皮的混合物。在使用酶提取物之前，测试冻干和不同的蛋白质沉淀方法以浓缩exo-PG和木聚糖酶。我们的结果表明，在-20 ℃下的乙醇沉淀是最合适的方法，获得的效率为50.2%和39.2%的外切-PG和木聚糖酶，分别。这种浓缩的蛋白质提取物用于澄清葡萄汁，在孵育40小时后，相对于初始比浊法降低了97.5%，这改善了用其它商业粗酶获得的澄清。我们的蛋白提取物中所含的exo-PG和木聚糖酶在不同温度、pH和不同阳离子存在下非常稳定。最后，exo-PG和木聚糖酶的部分纯化，通过阴离子交换层析上的DEAE-纤维素柱获得富集的35.5-和5.5-倍，分别超过培养上清。这些结果表明，通过该方法获得的具有高含量exo-PG和木聚糖酶的浓缩提取物在果汁和葡萄酒工业中具有良好的应用前景。(C)2010爱思唯尔有限公司版权所有。
Grape pomace, a residue produced in the wine industry, is becoming an environmental problem. By other hand enzymes extracts are products of great value in the wine making. In this work we evaluated applicability in must clarification of an enzyme extract produced by solid state fermentation on a mixture of grape pomace and orange peel. Before using the enzyme extracts, lyophilisation and different methods of protein precipitation were tested in order to concentrate exo-PG and xylanase. Our results showed that ethanol precipitation at -20 degrees C was the most adequate method, obtaining efficiencies of 50.2% and 39.2% for exo-PG and xylanase, respectively. This concentrated protein extract was used for clarification of must, giving a decrease of 97.5% respect to the initial turbidimetry after 40 h of incubation, which improved the clarification obtained with other commercial crude enzymes. Exo-PG and xylanase contained in our protein extracts were very stable at different temperatures, pH and in the presence of different cations. Finally, exo-PG and xylanase were partially purified by anion exchange chromatography on DEAE-cellulose column obtaining enrichments of 35.5-and 5.5-fold respectively over the culture supernatant. All these results suggested the promising application in juice and wine industries of the concentrated extracts with high levels of exo-PG and xylanase obtained by the proposed method. (C) 2010 Elsevier Ltd. All rights reserved.