Expression of Mina53, a product of a Myc target gene in mouse testis.
Expression of Mina53, a product of a Myc target gene in mouse testis.
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DOI：
10.1111/j.1365-2605.2005.00572.x
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发表时间：
2006-04
影响因子：
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通讯作者：
M. Tsuneoka;Y. Nishimune;K. Ohta;K. Teye;Hiromitsu Tanaka;M. Soejima;H. Iida;T. Inokuchi;H. Kimu
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文献类型：
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作者：
M. Tsuneoka;Y. Nishimune;K. Ohta;K. Teye;Hiromitsu Tanaka;M. Soejima;H. Iida;T. Inokuchi;H. Kimu
最近，我们发现了一个新的基因mina53（mina），它是癌蛋白Myc的直接转录靶点。Mina53蛋白在肿瘤细胞中高度表达，并在细胞增殖中发挥作用。在这里，我们报告了Mina53在小鼠睾丸中的表达，该睾丸含有增殖细胞并表达许多癌症相关基因。用新制备的Mina53单克隆抗体进行免疫组化研究，结果表明Mina53在精原细胞核中表达。Mina53在减数分裂前期的前细线期、细线期和偶线期细胞中也有表达，在粗线期早期的精母细胞中有微弱表达，而在粗线期晚期的精母细胞、精子细胞和成熟精子中不表达。Mina53的表达模式与增殖细胞核抗原（PCNA）的表达模式十分相似。用实验性隐睾动物睾丸组织进行检测，发现Mina53在未分化精原细胞中高表达，且PCNA阳性。这些结果表明，Mina53是显着表达在增殖，未分化的精原细胞，并在精子发生的减数分裂前期的精原细胞阶段的细胞增殖中发挥作用，但不是在减数分裂本身。
Recently we have identified a novel gene mina53 (mina), which is a direct transcriptional target of oncoprotein Myc. Mina53 protein was shown to be highly expressed in tumour cells and to play a role in cell proliferation. Here we report the expression of Mina53 in mouse testis, which contains proliferating cells and expresses many cancer-related genes. Immunohistochemical studies by using newly produced monoclonal antibody to Mina53 showed that Mina53 was expressed in the nuclei of spermatogonia. Mina53 was also expressed in meiotic prophase cells such as preleptotene, leptotene and zygotene, and weakly in early pachytene spermatocytes, but was absent in late pachytene spermatocytes, spermatids and mature sperm. The expression pattern of Mina53 was quite similar to that of proliferation cell nuclear antigen (PCNA). Using experimental cryptorchid testis, it was found that Mina53 was highly expressed in undifferentiated spermatogonia, which were PCNA-positive. These results suggest that Mina53 is prominently expressed in proliferating, undifferentiated spermatogonia, and plays a role in cell proliferation from the spermatogonial stage to the meiotic prophase in spermatogenesis, but not in meiotic divisions per se.