Expression of extracellular matrix genes:: Transforming growth factor (TGF)-β1 and ras in tibial fracture healing of lathyritic rats
Expression of extracellular matrix genes:: Transforming growth factor (TGF)-β1 and ras in tibial fracture healing of lathyritic rats
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DOI：
10.1016/s8756-3282(00)00359-8
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发表时间：
2000-10-01
期刊：
影响因子：
4.1
通讯作者：
Penttinen, RPK
中科院分区：
文献类型：
--
作者：
Ekholm, EC;Ravanti, L;Penttinen, RPK
由膳食氨基乙腈（AAN）诱导的实验性骨溶解症用于研究改变的细胞外基质对长骨愈合中结缔组织组分表达的影响。AAN抑制赖氨酰氧化酶，赖氨酰氧化酶是胶原交联前体形成所需的，并且还显示作为Ras的调节剂，在lavitic大鼠胫骨骨折发展的机械薄弱的骨痂组织与不规则的软骨和减少糖胺聚糖积累，软骨特异性蛋白质的过量（II型胶原、IX型胶原和X型胶原和聚集蛋白聚糖）在时间上在乳浊病愈伤组织中比在对照中表达得更宽，并且即使在纤维化和骨化阶段也观察到活跃的转录。软结缔组织仍然存在于2-和3周龄的LATERICAL胼胝体，可以解释升高的III型胶原蛋白，双糖蛋白聚糖，和核心蛋白聚糖mRNA水平。控制细胞生长和分化的转化生长因子（TGF）-β 1和c-Ha-ras在软骨阶段都被上调。TGF-β 1的最大表达早于ras在骨萎缩中的最大表达，（Bone 27：551-557; 2000）（C）2000，Elsevier Science Inc，保留所有权利。
Experimental osteolathyrism, induced by dietary aminoacetonitrile (AAN), was used to study the effect of altered extracellular matrix on the expression of connective tissue components in long bone healing, AAN inhibits lysyl oxidase, which is needed for the formation of collagen cross-link precursors, and is also shown to act as a regulator of Ras, Fractured tibias in lathyritic rats develop excessive amounts of mechanically weak callus tissue with irregular cartilage and reduced glycosaminoglycan accumulation, Cartilage-specific proteins (collagen types II, IX, and X and aggrecan) were expressed temporally much wider in lathyritic calluses than in the controls, and active transcription was observed even during the fibrous and ossifying stages. Soft connective tissue was still present in 2- and 3-week-old lathyritic calluses and could explain the elevated type III collagen, biglycan, and decorin mRNA levels. Both transforming growth factor (TGF)-beta 1 and c-Ha-ras, which control cell growth and differentiation, were upregulated during the cartilaginous stage, The maximal expression of TGF-beta 1 preceded that of ras in osteolathyrism, (Bone 27:551-557; 2000) (C) 2000 by Elsevier Science Inc, All rights reserved.