Nitric oxide modulates ozone-induced cell death, hormone biosynthesis and gene expression in Arabidopsis thaliana
Nitric oxide modulates ozone-induced cell death, hormone biosynthesis and gene expression in Arabidopsis thaliana
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DOI：
10.1111/j.1365-313x.2008.03756.x
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发表时间：
2009-04-01
期刊：
影响因子：
7.2
通讯作者：
Kangasjarvi, Jaakko
中科院分区：
文献类型：
--
作者：
Ahlfors, Reetta;Brosche, Mikael;Kangasjarvi, Jaakko
一氧化氮（NO）与活性氧（ROS）共同参与植物的各种逆境反应。我们已经使用臭氧（O-3）作为一种工具，引发ROS激活的胁迫反应，并激活植物叶片中的细胞死亡。在这里，我们已经调查的角色和相互作用的ROS和NO在O-3诱导的细胞死亡的诱导和调节。O-3处理诱导NO的快速积累，从保卫细胞开始，扩散到邻近的表皮细胞，最终移动到叶肉细胞。在随后的时间点，NO的产生与过敏反应（HR）样病变的形成相一致。NO供体硝普钠（SNP）和O-3单独诱导了大量的防御相关基因;然而，在联合治疗中，SNP减弱了O-3对水杨酸（SA）生物合成和其他防御相关基因的诱导。与此一致，SNP处理也降低了O-3诱导的SA积累。O-3-敏感突变体rcd 1被认为是一个NO过量生产;相反，Atnoa 1/rif 1（拟南芥一氧化氮相关1/抗抑制FSM 1），一个突变体与减少生产的NO，也是O-3敏感。这与结合O-3和NO供体SNP的实验一起表明，在O-3暴露期间，NO可以改变植物中的信号传导、激素生物合成和基因表达，并且需要功能性NO产生来进行适当的O-3响应。总之，NO是响应O-3的重要信号分子。
Nitric oxide (NO) is involved together with reactive oxygen species (ROS) in the activation of various stress responses in plants. We have used ozone (O-3) as a tool to elicit ROS-activated stress responses, and to activate cell death in plant leaves. Here, we have investigated the roles and interactions of ROS and NO in the induction and regulation of O-3-induced cell death. Treatment with O-3 induced a rapid accumulation of NO, which started from guard cells, spread to adjacent epidermal cells and eventually moved to mesophyll cells. During the later time points, NO production coincided with the formation of hypersensitive response (HR)-like lesions. The NO donor sodium nitroprusside (SNP) and O-3 individually induced a large set of defence-related genes; however, in a combined treatment SNP attenuated the O-3 induction of salicylic acid (SA) biosynthesis and other defence-related genes. Consistent with this, SNP treatment also decreased O-3-induced SA accumulation. The O-3-sensitive mutant rcd1 was found to be an NO overproducer; in contrast, Atnoa1/rif1 (Arabidopsis nitric oxide associated 1/resistant to inhibition by FSM1), a mutant with decreased production of NO, was also O-3 sensitive. This, together with experiments combining O-3 and the NO donor SNP suggested that NO can modify signalling, hormone biosynthesis and gene expression in plants during O-3 exposure, and that a functional NO production is needed for a proper O-3 response. In summary, NO is an important signalling molecule in the response to O-3.