Clustered cell migration: Modeling the model system of Drosophila border cells
Clustered cell migration: Modeling the model system of Drosophila border cells
复制标题
簇状细胞迁移：果蝇边缘细胞模型系统建模
DOI：
10.1016/j.semcdb.2019.11.010
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发表时间：
2020
影响因子：
7.3
通讯作者：
Starz-Gaiano, Michelle
中科院分区：
文献类型：
--
作者：
Peercy, Bradford E.;Starz-Gaiano, Michelle
在不同的发育环境中，某些细胞必须迁移以履行其角色。关于控制细胞迁移的遗传和物理特性，仍有许多问题没有答案。虽然单个细胞单独移动的最简单的情况已经得到了很好的研究，但在考虑细胞群如何一起移动时会出现额外的复杂性。集体迁移细胞的模型之间存在显着差异。我们探索了果蝇（Drosophila melanogasteregg chamber）迁移边缘细胞簇的实验模型，该模型适合于直接观察和精确的遗传操作。该系统涉及两个值得关注的特殊特征：边界细胞簇包含有限数量的需要协调的迁移和非迁移细胞，并且它们在异质的三维微环境中导航。首先，我们回顾了如何集群的运动边界细胞的指定和指导其迁移的化学信号和邻近组织的相互作用的物理影响。在第二部分中，我们研究了围绕活动集群的3D结构和周围微环境的问题，以了解集群大小和通过卵室的运动速度的限制。数学模型已经确定了足够的基因调控网络的规格，捕捉涌现的行为在体内的关键力量，最低限度的监管拓扑信号，并直接细胞行为的关键信号线索的分布。这种研究边界细胞的跨学科方法可能会揭示适用于不同类型细胞迁移事件的管理原则。
In diverse developmental contexts, certain cells must migrate to fulfill their roles. Many questions remain unanswered about the genetic and physical properties that govern cell migration. While the simplest case of a single cell moving alone has been well-studied, additional complexities arise in considering how cohorts of cells move together. Significant differences exist between models of collectively migrating cells. We explore the experimental model of migratory border cell clusters inDrosophila melanogasteregg chambers, which are amenable to direct observation and precise genetic manipulations. This system involves two special characteristics that are worthy of attention: border cell clusters contain a limited number of both migratory and non-migratory cells that require coordination, and they navigate through a heterogeneous three-dimensional microenvironment. First, we review how clusters of motile border cells are specified and guided in their migration by chemical signals and the physical impact of adjacent tissue interactions. In the second part, we examine questions around the 3D structure of the motile cluster and surrounding microenvironment in understanding the limits to cluster size and speed of movement through the egg chamber. Mathematical models have identified sufficient gene regulatory networks for specification, the key forces that capture emergent behaviors observedin vivo, the minimal regulatory topologies for signaling, and the distribution of key signaling cues that direct cell behaviors. This interdisciplinary approach to studying border cells is likely to reveal governing principles that apply to different types of cell migration events.