High-Throughput Screening of Chlorella Vulgaris Growth Kinetics inside a Droplet-Based Microfluidic Device under Irradiance and Nitrate Stress Conditions
High-Throughput Screening of Chlorella Vulgaris Growth Kinetics inside a Droplet-Based Microfluidic Device under Irradiance and Nitrate Stress Conditions
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DOI：
10.3390/biom9070276
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发表时间：
2019-07-01
期刊：
影响因子：
5.5
通讯作者：
Shafik, Hesham Mohamed
中科院分区：
文献类型：
--
作者：
Saad, Marwa Gamal;Dosoky, Noura Sayed;Shafik, Hesham Mohamed
生物燃料是一种生态友好的可再生燃料，可以从微藻中提取。生物质和脂质生产率的最大化被认为是藻类生物柴油生产的主要挑战。由于传统的分批培养是耗时的、空间的和试剂消耗的，具有应用许多重复的许多限制，微流体技术最近已经作为具有高通量重复性和再现性的替代低成本和有效的技术出现。微流控装置在藻类生物技术中的不同应用已被报道，包括细胞鉴定、分选、捕获和代谢筛选。在这项工作中，通过将小球藻封装在一个简单的基于液滴的微阵列装置中，在20、80和200 μ mol/m2/s的不同光强下结合17.6、8.8和4.4 mM的不同硝酸盐浓度，研究了小球藻的生长结果。将微流控装置内的普通培养物与常规分批培养方法进行比较。此外，还研究了光照和氮供应不足的联合胁迫对微藻代谢谱的影响。结果表明，微流控通道内小球藻生长的最佳培养条件为17.6 mM和80 μ mol/m2/s。
Biodiesel is an eco-friendly renewable fuel that can be derived from microalgae. Maximization of biomass and lipid productivities are considered the main challenges for algal biodiesel production. Since conventional batch cultures are time-, space-, and reagent-consuming with many restrictions to apply many replicates, microfluidic technology has recently emerged as an alternative low-cost and efficient technology with high throughput repeatability and reproducibility. Different applications of microfluidic devices in algal biotechnology have been reported, including cell identification, sorting, trapping, and metabolic screening. In this work, Chlorella vulgaris was investigated by encapsulating in a simple droplet-based micro-array device at different light intensities of 20, 80, and 200 mu mol/m(2)/s combined with different nitrate concentrations of 17.6, 8.8, and 4.4 mM. The growth results for C. vulgaris within microfluidic device were compared to the conventional batch culture method. In addition, the effect of combined stress of deficiencies in irradiance and nitrogen availability were studied to illustrate their impact on the metabolic profiling of microalgae. The results showed that the most optimum favorable culturing conditions for Chlorella vulgaris growth within the microfluidic channels were 17.6 mM and 80 mu mol/m(2)/s.