IMPROVED ISOLATED HEART PREPARATION FOR EXTERNAL ASSESSMENT OF MYOCARDIAL-METABOLISM
IMPROVED ISOLATED HEART PREPARATION FOR EXTERNAL ASSESSMENT OF MYOCARDIAL-METABOLISM
复制标题
DOI：
10.1152/ajpheart.1979.236.4.h644
复制
发表时间：
1979-01-01
影响因子：
--
通讯作者：
SOBEL, BE
中科院分区：
文献类型：
--
作者：
BERGMANN, SR;CLARK, RE;SOBEL, BE
To facilitate characterization of myocardial metabolism with the use of positron-emitting tracers, an isolated heart preparation designed to simulate physiological levels of flow and substrate extraction was developed. Isovolumically beating rabbit hearts were perfused retrograde at 60 mmHg with modified non-recirculating Krebs-Henseleit (KH) solution with or without washed sheep red blood cells at a hematocrit of 25 or 40 (KH-RBC25, KH-RBC40). Left ventricular (LV) systolic pressure averaged 63 mmHg with KH alone, 78 with KH-RBC25 and 98 with KH-RBC40. LV dP/dt, pressure-time index and O2 consumption increased in an analogous fashion. Fatty acid extraction increased from 1.5% with KH to 11.8% in hearts perfused with KH-RBC40, but net uptake was not increased markedly due to the associated differences in flow (4.6 ml .cntdot. g-1 .cntdot. min-1 with KH and 1.5 with KH-RBC40). Hearts perfused with erythrocyte-rich media exhibited functional and metabolic stability and less edema. The preparation avoids intrinsic limitations encountered with buffer-perfused hearts and should prove useful for characterization of cardiac metabolism with radiolabeled tracers relevant to positron emission transaxial tomography.
