ONSET OF PUBERTY IN FEMALE RAT - CHANGES IN PLASMA PROLACTIN, GONADOTROPINS, LUTEINIZING HORMONE-RELEASING HORMONE (LHRH), AND HYPOTHALAMIC LHRH CONTENT
ONSET OF PUBERTY IN FEMALE RAT - CHANGES IN PLASMA PROLACTIN, GONADOTROPINS, LUTEINIZING HORMONE-RELEASING HORMONE (LHRH), AND HYPOTHALAMIC LHRH CONTENT
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DOI：
10.1210/endo-98-3-630
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发表时间：
1976-01-01
期刊：
影响因子：
4.8
通讯作者：
MCCANN, SM
中科院分区：
文献类型：
--
作者：
OJEDA, SR;WHEATON, JE;MCCANN, SM
为了研究雌性大鼠青春期开始时荷尔蒙变化的顺序，在第 32 天和第 38 天之间通过断头处死未成熟的动物，并通过特异性放射免疫测定 (RIA) 测定促性腺激素、催乳素和 LHRH [luliberin，黄体生成素释放激素] 的血浆滴度以及 LHRH 的下丘脑含量。在整个青春期的10:00和16:00小时将动物斩首，记录子宫重量，并检查卵巢是否有排卵迹象。将阴道关闭的动物根据子宫状况分为发情前期(A)、发情早期(EP)和发情后期(LP)，子宫未受刺激、有液体扩张或气球状。阴道张开通常与排卵相关，并且在大多数情况下发生在动情晚期末期。在阴道打开后3天内对动物进行研究，并根据阴道细胞学进行分组。子宫重量作为雌激素分泌的指标，在A期较低，在EP期增加，在LP期达到峰值，此后下降。从 A 到 LP 下午，血浆 LH [促黄体生成素] 和 FSH [促卵泡素，卵泡刺激素] 较低。此时，子宫重量最大，两种促性腺激素急剧增加。第二天早上（发情期），LH（但 FSH 没有）恢复到基础值。 FSH在发情下午恢复到基础水平。在整个期间，血浆催乳素在早上较低，但在下午出现峰值，在LP时达到最大值，此后随着子宫重量的变化模式而下降。整个青春期血浆 LHRH 均较低，而下丘脑 LHRH 含量在发情早晨（阴道开放当天）下降。雌性大鼠青春期的开始可能是由雌激素分泌逐渐增加引起的，雌激素分泌在中枢神经系统垂体水平上起作用，引发促性腺激素和催乳素的排卵前类似发情期的激增。
To study the sequence of hormonal changes that accompany the onset of puberty in the female rat, immature animals were sacrificed by decapitation between days 32 and 38, and plasma titers of gonadotropins, prolactin and LHRH [luliberin, luteinizing hormone-releasing hormone] and the hypothalamic content of LHRH were determined by specific radioimmunoassays (RIA). Animals were decapitated at 10:00 and 16:00 h throughout the pubertal period, the uterine weight was recorded, and the ovaries were inspected for signs of ovulation. Animals with the vagina closed were grouped according to the condition of the uterus as anestrus (A), early proestrus (EP) and late proestrus (LP), the uterus being unstimulated, dilated with some fluid or ballooned, respectively. Vaginal opening was usually associated with ovulation and in most cases occurred at the end of the late proestrous phase. Animals were studied up to 3 days after vaginal opening and were grouped according to vaginal cytology. Uterine weight, taken as an index of estrogen secretion, was low during A, increased during EP, and reached a peak at LP, declining thereafter. Plasma LH [lutropin, luteinizing hormone] and FSH [follitropin, follicle stimulating hormone] were low from A until the afternoon of LP. At this time, uterine weight was maximal and both gonadotropins increased dramatically. The following morning (estrus), LH, but not FSH, had returned to basal values. FSH returned to basal levels on the afternoon of estrus. Plasma prolactin was low in the morning during the entire period, but showed peaks on the afternoons, which reached a maximum at LP and declined thereafter following the pattern of changes in uterine weight. Plasma LHRH was uniformly low throughout the entire pubertal period, whereas hypothalamic LHRH content declined on the morning of estrus (day of viginal opening). The onset of puberty in the female rat possibly is brought about by a gradual increase in estrogen secretion which, acting at the CNS-pituitary level, triggers a preovulatory proestrous-like surge of gonadotropins and prolactin.