Toward β-amino acid proteins:: Design, synthesis, and characterization of a fifteen kilodalton β-peptide tetramer
Toward β-amino acid proteins:: Design, synthesis, and characterization of a fifteen kilodalton β-peptide tetramer
复制标题
DOI：
10.1021/ja077245x
复制
发表时间：
2008-01-23
影响因子：
15
通讯作者：
Schepartz, Alanna
中科院分区：
文献类型：
--
作者：
Petersson, E. James;Schepartz, Alanna
28 个残基的“复古”β 肽被设计为自组装为四聚体，成功地重现了八聚体 β 肽束复合物 Zwit-1F。 Z28 是迄今为止合成的最大的 β 肽，通过线性微波辅助合成实现，分离产率 19%，纯度高。 Z28 形成了预期的四聚体复合物，并且在折叠方面比 Zwit-1F 更稳定、更协作。 Z28的成功设计和合成是迈向真正的R氨基酸蛋白的重要一步。
A 28 residue 'retro' beta-peptide has been designed to self-assemble as a tetramer, successfully recapitulating the octameric beta-peptide bundle complex Zwit-1F. Z28, the largest beta-peptide synthesized to date, was achieved in a linear, microwave-assisted synthesis in 19% isolated yield and high purity. Z28 forms the expected tetrameric complex and is more stable and cooperative in its folding than Zwit-1F. The successful design and synthesis of Z28 is an important step toward true R amino acid proteins.