In vitro evaluation of digestive enzyme inhibition and antioxidant effects of naked oat phenolic acid compound (OPC)
In vitro evaluation of digestive enzyme inhibition and antioxidant effects of naked oat phenolic acid compound (OPC)
复制标题
裸燕麦酚酸化合物（OPC）消化酶抑制和抗氧化作用的体外评价
DOI：
10.1111/ijfs.14504
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发表时间：
2020-01
影响因子：
3.3
通讯作者：
Xiao Jianbo
中科院分区：
文献类型：
--
作者：
Yang Zhihao;Qin Chuan;Weng Peifang;Zhang Xin;Xia Qiang;Wu Zufang;Liu Lianliang;Xiao Jianbo
研究了裸燕麦酚酸类化合物（OPC）对消化酶的抑制作用及抗氧化性能。从乙酸乙酯馏分、正丁醇馏分和水相馏分中分离出游离和结合酚酸。研究了OPC与主要消化酶（α-淀粉酶、α-葡萄糖苷酶、胃蛋白酶和胰蛋白酶）的相互作用。结果表明，半纯化的结合酚酸（通过AB-8柱半纯化）具有竞争性α-葡萄糖苷酶抑制剂，而有机提取物级分的OPC表现出混合抑制剂的特征。结合酚正丁醇组分（IC 50 =98.39±0.89 μg/mL）对1，1-二苯基-2-苦基肼基（DPPH）的抑制能力最强。另外，裸燕麦酚类化合物正丁醇提取物的淀粉水解度显著低于其他组分。结果提示，OPC可能是一种潜在的控制餐后血糖的健康因子，其作用机制可能与抗消化、抗氧化以及与糖尿病相关淀粉相互作用有关。
This research was focused on digestive enzyme inhibition and antioxidant properties of naked oat phenolic acid compound (OPC). Free and bound phenolic acid were separated from ethyl acetate fraction, n-butanol fraction and aqueous fraction. The interactions between OPC and main digestive enzymes (α-amylase, α-glucosidase, pepsin and trypsin) were studied. It was shown that the semi-purified bound phenolic acid (semi-purified by AB-8 column) has a competitive alpha-glucosidase inhibitor, while OPC of the organic extract fraction exhibited the characteristics of a mixed inhibitor. Bound phenolic n-butanol fraction (IC50=98.39±0.89 µg/mL) had the strongest ability to scavenge the 1,1-diphenyl-2-picrylhydrazyl (DPPH). Additionally, the starch hydrolysis degree of n-butanol extraction naked oat phenolic compound was significantly lower than other fractions in vitro. The integrated results suggested that OPC could be considered as potential healthy factor to control postprandial blood glucose and the mechanism maybe via anti-digestion, antioxidation and interaction with diabetes-related starch.
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