A Subcutaneous Biochip for Remote Monitoring of Human Metabolism: Packaging and Biocompatibility Assessment
A Subcutaneous Biochip for Remote Monitoring of Human Metabolism: Packaging and Biocompatibility Assessment
复制标题
用于远程监测人体新陈代谢的皮下生物芯片：包装和生物相容性评估
作者：
A. Cavallini;T. R. Jost;S. Ghoreishizadeh;J. Olivo;M. O. de Beeck;B. Gorissen;F. Grassi;G. De Micheli;S. Carrara
本文是2012年IEEE传感器会议上发表的会议论文“开发用于远程监测人体代谢的高度集成皮下生物芯片”的扩展版本，并提供了关于完全植入式生物传感器阵列的组装、包装和短期体外和体内生物相容性评价的数据。该设备集成了三个构建模块：1）多电极平台; 2）感应线圈; 3）集成电路。整个系统的尺寸为2.2 mm × 2.2 mm × 15 mm。使用Parylene C的保形涂层防止电子组件腐蚀和潜在有害物质泄漏，同时使用医用级硅胶的外包装创建适合植入的软外壳。生物相容性实验在考虑的7天时间内没有显示出体外细胞毒性，而7天和30天体内移植之间的比较显示出炎症反应随时间的显著减少，表明正常的宿主恢复。
This paper represents the extended version of the conference paper “Developing highly-integrated subcutaneous biochips for remote monitoring of human metabolism” presented at the IEEE Sensors Conference 2012, and presents data on assembly, packaging and short term in vitro and in vivo biocompatibility evaluation of a fully implantable biosensor array. The device was realized integrating three building blocks: 1) a multielectrode platform; 2) an inductive coil; and 3) an integrated circuit. The entire system measures 2.2 mm × 2.2 mm × 15 mm. Corrosion of electronic components and leaking of potentially hazardous substances in the body is prevented with a conformal coating of Parylene C, while an outer package of medical grade silicone was employed to create a soft shell suitable for implantation. Biocompatibility experiments did not show in vitro cytotoxicity in the considered period of 7 days, while comparison between 7 and 30 days in vivo implantations showed significant reduction of the inflammatory response in time, suggesting normal host recovery.