A medium supplemented with vegetable extract used for cellulose production of Rhodococcus SP. MI 2
A medium supplemented with vegetable extract used for cellulose production of Rhodococcus SP. MI 2
复制标题
补充有植物提取物的培养基，用于红球菌 SP 的纤维素生产。
DOI：
10.14393/bj-v34n3a2018-36954
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发表时间：
2018
影响因子：
0.5
通讯作者：
Nathakan Jaturonlak
中科院分区：
文献类型：
--
作者：
Somporn Tanskul;Tassanaporn Damthongsen;Nathakan Jaturonlak
：在这项研究中，筛选了最合适的蔬菜提取物作为红球菌属纤维素生产的非常规营养源。 MI 2.SH培养基或合成培养基用作常规或对照培养基。 Cha-poo (Piper sarmentosum Roxb.) 和甘薯 (Ipomoea batatas Lam.) 是 14 种蔬菜提取物中的 2 种，被选为培养基补充剂。红球菌属MI 2 在补充有 Cha-poo 提取物的培养基中纤维素产量最高。室温（25℃）静态条件下添加茶普提取物的培养基中的最佳培养条件为接种量5%（v v -1）、培养时间6天、pH 3、3%蔗糖和0.5%（NH 4 ） 2 SO 4 。补充了Cha-poo提取物的培养基中的纤维素产量比优化前获得的纤维素产量（6天期间6.83 g L -1 ）增加了约3倍（6天期间2.39 g L -1 ）。补充有Cha-poo提取物的培养基的成本是SH培养基（培养基的1.9 USD L -1 ）的四分之一（培养基的0.5 USD L -1 ）。红球菌属产生的纤维素微纤维的结构。通过SEM观察到，补充有Cha-poo提取物的培养基中的MI 2比补充有甘薯提取物的培养基中产生的原纤维更大、更不拥挤。此外，前者的微原纤维有许多蜂巢状的结，而后者的微原纤维周围有外套膜状的结。
: In this study, the most suitable vegetable extract was screened to use as non-conventional nutrient sources for cellulose production of Rhodococcus sp. MI 2. SH medium or a synthetic medium was used as a conventional or control medium. Cha-poo ( Piper sarmentosum Roxb.) and sweet potato ( Ipomoea batatas Lam.) were 2 out of 14 vegetable extracts chosen as medium supplements. Rhodococcus sp. MI 2 gave the highest cellulose yield in a medium supplemented with Cha-poo extract. The optimum culture conditions in the medium supplemented with Cha-poo extract at room temperature (25 o C) under static condition were 5% (v v -1 ) inoculum size, a 6 -day -incubation period, pH 3, 3% sucrose, and 0.5% (NH 4 ) 2 SO 4 . The cellulose yield in the medium supplemented with Cha-poo extract was increased about 3 times (6.83 g L -1 during 6 days) higher than that obtained before optimizing (2.39 g L -1 during 6 days). The medium supplemented with Cha-poo extract cost one quarter (0.5 USD L -1 of medium) of the SH medium (1.9 USD L -1 of medium). The structure of the microfibrils of cellulose produced by Rhodococcus sp. MI 2 in a medium supplemented with Cha-poo extract observed by SEM had larger, less crowded fibrils than those produced in the medium supplemented with sweet potato extract. In addition, the microfibrils of the former had many beehive shaped knots whereas those of the latter had mantle-like surrounding the fibrils.
影响因子：
11.2
作者：
Kurosumi, Akihiro;Sasaki, Chizuru;Nakamura, Yoshitoshi
通讯作者：
Nakamura, Yoshitoshi