Hierarchical assembly of centriole subdistal appendages via centrosome binding proteins CCDC120 and CCDC68.
Hierarchical assembly of centriole subdistal appendages via centrosome binding proteins CCDC120 and CCDC68.
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通过中心体结合蛋白 CCDC120 和 CCDC68 分层组装中心粒远端附属物
DOI：
10.1038/ncomms15057
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发表时间：
2017-04-19
影响因子：
16.6
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Huang N;Xia Y;Zhang D;Wang S;Bao Y;He R;Teng J;Chen J
在动物细胞中，中心体是微管的主要组织中心，微管在中心体中成核和锚定。中心粒近远侧附属物（SDAs）是间期细胞中微管锚的关键结构，但SDAs的组成和组装机制尚不清楚。在这里，我们揭示了中心体结合蛋白、包含卷曲螺旋结构域（CCDC）120和CCDC 68是人类细胞中分层SDA组装所需的两种新型SDA组分。CCDC120通过ODF 2锚定到SDA，并通过其N末端的不同卷曲螺旋结构域将CEP 170和Ninein招募到中心体。CCDC68是一种与CEP170相互作用的蛋白质，它与CCDC120竞争将CEP170募集到SDA中。此外，CCDC 120和CCDC 68是中心体微管锚定所必需的。我们的研究结果阐明了人类间期细胞中中心粒SDA分级组装和微管锚定的分子基础。
In animal cells, the centrosome is the main microtubule-organizing centre where microtubules are nucleated and anchored. The centriole subdistal appendages (SDAs) are the key structures that anchor microtubules in interphase cells, but the composition and assembly mechanisms of SDAs are not well understood. Here, we reveal that centrosome-binding proteins, coiled-coil domain containing (CCDC) 120 and CCDC68 are two novel SDA components required for hierarchical SDA assembly in human cells. CCDC120 is anchored to SDAs by ODF2 and recruits CEP170 and Ninein to the centrosome through different coiled-coil domains at its N terminus. CCDC68 is a CEP170-interacting protein that competes with CCDC120 in recruiting CEP170 to SDAs. Furthermore, CCDC120 and CCDC68 are required for centrosome microtubule anchoring. Our findings elucidate the molecular basis for centriole SDA hierarchical assembly and microtubule anchoring in human interphase cells.