Isolation and characterization of Neurospora crassa nucleoside diphosphate kinase NDK-1.
Isolation and characterization of Neurospora crassa nucleoside diphosphate kinase NDK-1.
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粗糙脉孢菌核苷二磷酸激酶 NDK-1 的分离和表征。
DOI：
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
K. Hasunuma
中科院分区：
文献类型：
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作者：
Yasunobu Ogura;Y. Yoshida;Kazuya Ichimura;Chito Aoyagi;N. Yabe;K. Hasunuma
我们以前曾报道过蓝光照射后粗糙脉孢菌中15 kDa蛋白的磷酸化增加。用DEAE-纤维素柱、Blue-Sepharose柱、SP-Sepharose柱和Mono-Q柱对15 kDa蛋白进行了纯化，氨基酸序列分析表明该蛋白与核苷二磷酸激酶同源，并具有核苷二磷酸激酶活性。从菌丝体基因文库和基因组文库中分离到一个编码粗面核苷二磷酸核苷二磷酸的基因NDK-1。NDK-1的氨基酸序列与15 kDa蛋白的氨基酸序列完全一致。Northern印迹分析表明，蓝光信号转导的关键因子WC-1和WC-2不在转录水平上调控NDK-1。NDK-1对髓鞘碱性蛋白也表现出快速的自磷酸化活性和蛋白激酶活性，Km值为0.36 mm。这些结果表明，NDK-1通过磷酸化蛋白质而发挥信号转导作用。
We have previously reported that phosphorylation of a 15-kDa protein increased after blue-light irradiation in Neurospora crassa. In this study, the 15-kDa protein was purified using four columns; DEAE-cellulose, Blue-Sepharose, SP-Sepharose and Mono Q. The 15-kDa protein was shown to be homologous with nucleoside diphosphate kinase by amino acid sequencing and was also shown to possess nucleoside diphosphate kinase activity. A gene encoding N. crassa nucleoside diphosphate kinase, ndk-1, was isolated from the mycelial cDNA and genomic libraries. The deduced amino acid sequence of NDK-1 was identical to that of the 15-kDa protein. Northern blot analysis suggested that WC-1 and WC-2, the key factors of blue-light signal transduction in N. crassa, did not regulate NDK-1 at the transcriptional level. NDK-1 also showed rapid autophosphorylation activity and protein kinase activity against myelin basic protein with a Km value of 0.36 mM. These results suggest that NDK-1 acts as a signal transducer by phosphorylating proteins.
DOI：
10.1073/pnas.81.5.1470
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
YELTON, MM;HAMER, JE;TIMBERLAKE, WE
通讯作者：
TIMBERLAKE, WE
通过质谱法鉴定牛髓磷脂碱性蛋白中苏氨酸 97 作为丝裂原激活蛋白激酶的特异性磷酸化位点。
DOI：
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发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Erickson,AK;Payne,DM;Martino,PA;Rossomando,AJ;Shabanowitz,J;Weber,MJ;Hunt,DF;Sturgill,TW
通讯作者：
Sturgill,TW
影响因子：
5.6
作者：
Roger L. Williams;D. Oren;J. Muñoz-Dorado;S. Inouye;M. Inouye;Edward A Arnold
通讯作者：
Roger L. Williams;D. Oren;J. Muñoz-Dorado;S. Inouye;M. Inouye;Edward A Arnold