Recognition-driven chemical labeling of endogenous proteins in multi-molecular crowding in live cells
Recognition-driven chemical labeling of endogenous proteins in multi-molecular crowding in live cells
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DOI：
10.1039/c7cc07177a
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发表时间：
2017-11-14
影响因子：
4.9
通讯作者：
Hamachi, Itaru
中科院分区：
文献类型：
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作者：
Amaike, Kazuma;Tamura, Tomonori;Hamachi, Itaru
内源性蛋白质标记是研究活细胞中蛋白质真实功能的最有价值的方法之一。然而，多分子拥挤条件，例如那些发生在活细胞中的条件，阻碍了感兴趣蛋白（POI）的高选择性化学标记。我们在这里描述了分子识别与化学反应的有效耦合如何对选择性蛋白质标记至关重要。识别驱动的蛋白质标记是通过含有蛋白质（识别）配体、报告标记和反应片段的合成标记试剂进行的。即使在活细胞条件下，POI的分子识别也可以大大提高反应动力学和蛋白质选择性。在这篇综述中，我们也简要地讨论了这种内源性蛋白质的选择性化学标记如何在化学和生物学的界面上有各种应用。
Endogenous protein labeling is one of the most invaluable methods for studying the bona fide functions of proteins in live cells. However, multi-molecular crowding conditions, such as those that occur in live cells, hamper the highly selective chemical labeling of a protein of interest (POI). We herein describe how the efficient coupling of molecular recognition with a chemical reaction is crucial for selective protein labeling. Recognition-driven protein labeling is carried out by a synthetic labeling reagent containing a protein (recognition) ligand, a reporter tag, and a reactive moiety. The molecular recognition of a POI can be used to greatly enhance the reaction kinetics and protein selectivity, even under live cell conditions. In this review, we also briefly discuss how such selective chemical labeling of an endogenous protein can have a variety of applications at the interface of chemistry and biology.