Chimeras between parthenogenetic or androgenetic blastomeres and normal embryos: allocation to the inner cell mass and trophectoderm.
Chimeras between parthenogenetic or androgenetic blastomeres and normal embryos: allocation to the inner cell mass and trophectoderm.
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DOI：
10.1016/s0012-1606(89)80028-4
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发表时间：
1989-02
影响因子：
2.7
通讯作者：
James Alexander Thomson;Davor Solter
中科院分区：
文献类型：
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作者：
James Alexander Thomson;Davor Solter
一系列的嵌合体是通过注射单个正常的，pathenogenetic，或雄核发育的卵裂球携带转基因标记下的非转基因八细胞胚胎的透明膜。将这些嵌合体培养至囊胚期并切片，用原位杂交检测转基因成分。在正常、孤雌生殖和雄核发育嵌合体中，对内细胞团（ICM）、滋养外胚层或ICM和滋养外胚层均具有转基因贡献的嵌合囊胚数量无统计学显著差异。由于雄激素和孤雌生殖细胞存在于嵌合体中，在高频率的ICM和滋养外胚层在囊胚阶段，但不是在类似的嵌合体在原肠胚形成后期，这些细胞必须不响应正常的发育信号后，囊胚形成和前原肠胚形成后期。
A series of chimeras was generated by injecting single normal, pathenogenetic, or androgenetic blastomeres carrying transgenic markers under the zona pellucida of nontransgenic eight-cell embryos. These chimeras were cultured to the blastocyst stage and sectioned, and the transgenic component was detected byin situhybridization. No statistically significant different was found among the normal, parthenogenetic, and androgenetic chimeras in the number of chimeric blastocysts with a transgenic contribution to the inner cell mass (ICM), the trophectoderm, or both the ICM and trophectoderm. Since androgenetic and parthenogenetic cells were present in chimeras at a high frequency in both the ICM and trophectoderm at the blastocyst stage, but not in similar chimeras at late gastrulation, these cells must not respond normally to developmental signals subsequent to blastocyst formation and prior to late gastrulation.