Development of CD8α/α and CD8α/β T cells in major histocompatibility complex class I-deficient mice
Development of CD8α/α and CD8α/β T cells in major histocompatibility complex class I-deficient mice
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DOI：
10.1084/jem.190.6.881
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发表时间：
1999-09-20
影响因子：
15.3
通讯作者：
Janeway, CA
中科院分区：
文献类型：
--
作者：
Das, G;Janeway, CA
外周CD8(+) T细胞主要使用CD8 α / β，其发育主要依赖于H-2(b)小鼠的主要组织相容性复合体（MHC） I类蛋白K-b和D-b。在本报告中，我们已经证明CD8 α / β tcr - α / β细胞在淋巴器官以及肠上皮内淋巴细胞（iIELs）中的发育依赖于MHC I类K-b和D-b蛋白。相比之下，tcr - α / β CD8 α / α细胞主要存在于iels中，其数量在(KDb)-D-b双敲除小鼠中不受影响。iels中的大多数tcr - γ / δ细胞也携带CD8 α / α，在(KDb)- d -b -/-小鼠中也不受影响。在β 2微球蛋白（β 2m）缺乏的小鼠中，所有的tcr - α / β CD8 α / α和CD8 α / β T细胞消失，但tcr - γ / δ细胞不受β 2m缺失的影响。
Peripheral CD8(+) T cells mainly use CD8 alpha/beta, and their development is mainly dependent on the major histocompatibility complex (MHC) class I proteins K-b and D-b in H-2(b) mice. In this report, we have shown that the development of CD8 alpha/beta TCR-alpha/beta cells in lymphoid organs as well as in intestinal intraepithelial lymphocytes (iIELs) is dependent on the MHC class I K-b and D-b proteins. In contrast, TCR-alpha/beta CD8 alpha/alpha cells are found mainly in iIELs, and their numbers are unaffected in (KDb)-D-b double knockout mice. Most of the TCR-gamma/delta cells in the iIELs also bear CD8 alpha/alpha, and they are also unaffected in (KDb)-D-b -/- mice. In beta 2-microglobulin (beta 2m)-deficient mice, all of the TCR-alpha/beta CD8 alpha/alpha and CD8 alpha/beta T cells disappear, but TCR-gamma/delta cells are unaffected by the absence of beta 2m.