An acutely lethal simian immunodeficiency virus stimulates expansion of V beta 7- and V beta 14-expressing T lymphocytes.
An acutely lethal simian immunodeficiency virus stimulates expansion of V beta 7- and V beta 14-expressing T lymphocytes.
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一种急性致命的猿猴免疫缺陷病毒会刺激表达 V beta 7 和 V beta 14 的 T 淋巴细胞的扩增。
DOI：
10.1073/pnas.91.16.7501
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发表时间：
1994
影响因子：
11.1
通讯作者：
Letvin,NL
中科院分区：
文献类型：
--
作者：
Chen,ZW;Kou,ZC;Shen,L;Regan,JD;Lord,CI;Halloran,M;Lee-Parritz,D;Fultz,PN;Letvin,NL
SIVsmmPBj 14是一种从猪尾猕猴中分离的变异猿免疫缺陷病毒，在体外刺激猕猴T淋巴细胞增殖，并诱导猕猴急性致死性疾病，部分特征为淋巴结病和脾肿大。为了确定SIVsmmPBj 14是否表现出超抗原样活性，使用基于PCR的定量方法进行T细胞受体V β库的体外和体内研究。然而，在体外植物血凝素刺激猕猴外周血淋巴细胞没有引起T细胞受体V β库的扰动，SIVsmmPBj14刺激表达V β 7和V β 14基因家族的CD4+和CD8+ T淋巴细胞亚群的扩增。这种V β 7和V β 14扩增可以通过多重RNA酶保护测定来证实。此外，同样的淋巴细胞亚群的扩增也被检测到在外周血淋巴细胞和淋巴结细胞的病毒感染的猕猴。这些观察结果表明，SIVsmmPBj14介导的V β扩增可能有助于在猕猴中诱导急性致死性疾病。
SIVsmmPBj14, a variant simian immunodeficiency virus isolated from a pig-tailed macaque, stimulates the proliferation of macaque T lymphocytes in vitro and induces an acutely lethal disease in macaques characterized, in part, by lymphadenopathy and splenomegaly. To determine whether SIVsmmPBj14 exhibits superantigen-like activity, in vitro and in vivo studies of T-cell receptor V beta repertoire were undertaken using PCR-based quantitative methods. Whereas in vitro phytohemagglutinin stimulation of macaque peripheral blood lymphocytes did not cause a perturbation of T-cell receptor V beta repertoire, SIVsmmPBj14 stimulated the expansion of both CD4+ and CD8+ T-lymphocyte subpopulations expressing the V beta 7 and V beta 14 gene families. Such V beta 7 and V beta 14 expansions could be confirmed by a multiple RNase protection assay. Furthermore, the expansion of the same lymphocyte subpopulations was also detected in peripheral blood lymphocytes and lymph node cells of virus-infected macaques. These observations suggest that SIVsmmPBj14-mediated V beta expansion may contribute to the induction of an acutely lethal disease in macaques.