AtCYP20‐2 is an auxiliary protein of the chloroplast NAD(P)H dehydrogenase complex
AtCYP20‐2 is an auxiliary protein of the chloroplast NAD(P)H dehydrogenase complex
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AtCYP20-2 是叶绿体 NAD(P)H 脱氢酶复合物的辅助蛋白
DOI：
10.1016/j.febslet.2009.06.031
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发表时间：
2009
期刊：
影响因子：
3.5
通讯作者：
E. Aro
中科院分区：
文献类型：
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作者：
Sari Sirpiö;Maija Holmström;N. Battchikova;E. Aro
AtCYP 20 -2是类囊体腔中16种亲免蛋白之一。AtCYP 20 -2中异构酶结构域的存在、AtCYP 20 -2在基质膜中的富集以及它在天然凝胶中与NAD（P）H脱氢酶（NDH）的共迁移提供了AtCYP 20 -2是NDH辅助蛋白的证据。当研究不同的NDH突变体时，发现AtCYP 20 -2强烈减少，特别是在NDH的膜结构域缺陷的突变体中，从而表明在NDH疏水结构域的组装中的作用。然而，AtCYP 20 -2的缺乏并没有导致NDH的严重功能障碍，这表明内腔亲免素的功能冗余。
AtCYP20-2 is one of 16 immunophilins in thylakoid lumen. The presence of the isomerase domain in AtCYP20-2, an enrichment of AtCYP20-2 in the stroma membranes and it’s co-migration with NAD(P)H dehydrogenase (NDH) in native gels provide evidence that AtCYP20-2 is an auxiliary protein of NDH. When different NDH mutants were studied, AtCYP20-2 was found to be strongly reduced especially in mutants deficient in the membrane domain of NDH, thus suggesting a role in the assembly of NDH hydrophobic domain. Lack of AtCYP20-2, however, did not lead to severe malfunction of NDH, indicating redundancy in the function of lumenal immunophilins.
K.Asada：“通过监测 P700 和叶绿素荧光的氧化还原变化，在完整叶子中检测到从基质成分到系统间链的电子流和玉米叶绿体中的循环电子流”
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