Protocollagen Proline Hydroxylase Activity in Rat Heart During Experimental Cardiac Hypertrophy
Protocollagen Proline Hydroxylase Activity in Rat Heart During Experimental Cardiac Hypertrophy
复制标题
实验性心肌肥厚大鼠心脏中Protoline脯氨酸羟化酶活性
作者：
S. Lindy;H. Turto;J. Uitto
在大鼠腹主动脉下缩窄，造成心肌肥厚。主动脉缩窄后2天左心室重量显著升高，11天时比假手术动物高19%。测定心肌中参与胶原生物合成的原胶原脯氨酸羟化酶（PPH）活性和羟脯氨酸含量。PPH活性在主动脉缩窄后2天增加，此后下降，11天仍高于对照水平。与对照组相比，肥厚心肌中左心室的羟脯氨酸含量在11天时显著增加。目前的结果表明，在心肌肥厚的早期结缔组织激活发生。后来，这导致结缔组织积聚。增加的胶原蛋白含量可以支持心肌收缩对抗收缩超负荷。
Cardiac hypertrophy was produced in rats by constricting the abdominal aorta subdiaphragmatically. The weight of the left ventricle was significantly elevated 2 days after aortic constriction and was 19% higher than in shamoperated animals at 11 days. The activity of protocollagen proline hydroxylase (PPH), an enzyme participating in collagen biosynthesis, and the hydroxyproline content of the heart muscle were determined. PPH activity was increased at 2 days after aortic constriction and declined thereafter, being still above the control level at 11 days. The hydroxyproline content of the left ventricle was significantly increased at 11 days in hypertrophied heart muscle compared to controls. The present results suggest that in cardiac hypertrophy an early connective tissue activation occurs. Later, this leads to connective tissue accumulation. The increased collagen content may give support to the cardiac muscle contracting against systolic overload.