NEURONAL LOCALIZATION OF CD38 ANTIGEN IN THE HUMAN BRAIN
NEURONAL LOCALIZATION OF CD38 ANTIGEN IN THE HUMAN BRAIN
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DOI：
10.1016/0006-8993(95)00885-t
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发表时间：
1995-10-30
期刊：
影响因子：
2.9
通讯作者：
IKEDA, K
中科院分区：
文献类型：
--
作者：
MIZUGUCHI, M;OTSUKA, N;IKEDA, K
CD 38是一种淋巴细胞分化抗原，参与环ADP-核糖介导的第二信使系统。我们提供了免疫化学和免疫组织化学证据CD 38在成人大脑中的表达。我们使用了六个多克隆抗体对合成的CD 38多肽，除了四个单克隆抗体已经可用。免疫亲和纯化脑组织提取物后，Western blotting可检测到脑CD 38。免疫过氧化物酶染色定位CD 38免疫反应性的胞体和树突的许多神经元，如小脑浦肯野细胞，这意味着CD 38参与中枢神经系统神经元内的信号转导。
CD38 is a lymphocyte differentiation antigen which is involved in the cyclic ADP-ribose-mediated second messenger system. We provide immunochemical and immunohistochemical evidence for the expression of CD38 in the adult human brain. We used six polyclonal antibodies against synthetic CD38 polypeptides, in addition to four monoclonal antibodies already available. Brain CD38 was detectable py Western blotting after immunoaffinity Purification of the brain extracts. Immunoperoxidase staining localized CD38 immunoreactivity to the perikarya and dendrites of many neurons, such as the cerebella Purkinje cells, implying that CD38 is involved in the signal transduction within the central nervous system neurons.