Effect of pentobarbital on cyanide-induced tremors in mice and calcium accumulation in PC12 cells.
Effect of pentobarbital on cyanide-induced tremors in mice and calcium accumulation in PC12 cells.
复制标题
戊巴比妥对氰化物引起的小鼠震颤和 PC12 细胞钙积累的影响。
DOI：
10.1016/0006-2952(87)90064-5
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发表时间：
1987
影响因子：
5.8
通讯作者：
Isom,GE
中科院分区：
文献类型：
--
作者：
Johnson,JD;Conroy,WG;Isom,GE
震颤、癫痫发作和中枢病变是急性氰化物中毒的常见表现[1]。我们的实验室最近报道，氰化物诱导的震颤可能与细胞内钙的神经元调节的改变有关[2，3]。由于ATP的可用性降低，氰化物产生胞质Caz+的积累，ATP是维持能量依赖性钙稳态过程所必需的。胞浆Ca*+升高可诱导神经递质释放，导致全身性CNS兴奋，表现为惊厥或震颤。已确定巴比妥类药物对神经递质释放[4]以及其他钙依赖性神经元过程的抑制作用[5，6]。已报道了戊巴比妥降低氰化物诱导惊厥发生率的能力[7]，但尚未进行更详细的研究。本研究的目的是描述戊巴比妥对氰化物诱导的小鼠震颤的作用，并将这些作用与PC12细胞胞浆Ca2+的氰化物蓄积相关联。
Tremors, seizures and central lesions are common manifestations of acute cyanide poisonings [l]. Our laboratory recently reported that cyanide-induced tremors may be related to alterations in neuronal regulation of intracellular calcium [2, 3]. Cyanide produces accumulation of cytosolic Caz+ as a result of decreased availability of ATP which is necessary for maintenance of energy-dependent calcium homeostatic processes. Elevated cytosolic Ca*+ can induce the release of neurotransmitters, leading to generalixed CNS excitation manifested as convulsions or tremors. The inhibitory actions of barbiturates on neurotransmitter release [4], as well as other calcium-dependent neuronal processes, have been established [5, 6]. The ability of pentobarbital to decrease the incidence of cyanideinduced convulsions has been-reported [7], but a more detailed study has not been conducted. The objective of this study was to characterixe the effect of pentobarbital on cyanide-induced tremors in mice and to correlate these efforts with cyanide accumulation of cytosolic Ca2+ in PC12