De Novo Structural Modeling and Conserved Epitopes Prediction of Zika Virus Envelop Protein for Vaccine Development
De Novo Structural Modeling and Conserved Epitopes Prediction of Zika Virus Envelop Protein for Vaccine Development
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DOI：
10.1089/vim.2016.0033
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发表时间：
2016-09-01
期刊：
影响因子：
2.2
通讯作者：
Ahmed, Bilal
中科院分区：
文献类型：
--
作者：
Ashfaq, Usman Ali;Ahmed, Bilal
寨卡病毒（寨卡V型）是一种阳性单链RNA病毒，通过蚊虫叮咬传播。寨卡病毒包膜蛋白具有抗原性，参与病毒颗粒的融合和进入细胞。到目前为止，还没有针对寨卡病毒的疫苗和抗病毒药物。因此，有必要开发一种针对寨卡病毒的疫苗。这项研究旨在预测B细胞和T细胞表位，从而有助于诊断和设计针对这种新出现的威胁的疫苗。为此，预测了从12个不同国家采集的寨卡病毒基因组中保守的几个B细胞和T细胞表位。在主要组织相容性复合体（MHC） I类情况下，肽qtlltpvgrl和MHC II类情况下，IRCIGVSNRDFV在T细胞表位中具有高抗原性。通过分子对接研究B细胞表位与HLA-B7的相互作用。然而，这些预测的表位可以在设计寨卡病毒疫苗中发挥建设性作用。
Zika virus (Zika V) is a positive single-stranded RNA virus that is transmitted by mosquito bites. Zika V Envelop protein is antigenic and is involved in fusion and entry of viral particles into the cell. Till date, there is no vaccine and antiviral drug available against Zika V. Thus, there is a need to develop a vaccine against Zika V. This study was designed for the prediction of B cell and T cell epitopes that can be helpful in diagnosis and vaccine designing against this emerging threat. For this purpose, several B cell and T cell epitopes were predicted that are conserved among Zika virus genomes taken from 12 different countries. Peptides QTLTPVGRL, in case of major histocompatibility complex (MHC) class I, and IRCIGVSNRDFV, in case ofMHC class II, are highly antigenic among T cell epitopes. Molecular docking was performed to study the interactions of B cell epitopes with HLA-B7. However, these predicted epitopes could play a constructive role in designing of a vaccine against Zika V.