Anthocyanin composition and expression analysis of anthocyanin biosynthetic genes in kidney bean pod
Anthocyanin composition and expression analysis of anthocyanin biosynthetic genes in kidney bean pod
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芸豆荚花色苷组成及花色苷生物合成基因表达分析
DOI：
10.1016/j.plaphy.2015.10.019
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发表时间：
2015-12-01
影响因子：
6.5
通讯作者：
Hu, Zongli
中科院分区：
文献类型：
--
作者：
Hu, Jingtao;Chen, Guoping;Hu, Zongli
芸豆（Phaseolus vulgaris L.）是世界范围内种植和消费的重要膳食豆类作物。选择紫色品种紫霸王和绿色品种春秋，主要区别在于荚果表皮颜色。采用HPLC-ESI-MS/MS方法鉴定紫霸王豆荚皮中主要的花青素为马来糖苷3,5 -二葡糖苷。与其他材料相比，紫豆荚皮中PvMYB1、PvMYB2、PvTT8-1等3个调控基因和大部分结构基因的表达显著上调。与未包袋荚果相比，包袋荚果紫色皮中所有调控基因和大部分生物合成基因的表达水平也显著降低。上述结果表明，PvMYB1、PvMYB2和PvTT8-1可能在紫芸豆豆荚中大部分花青素生物合成基因的转录激活中起关键作用。(C) 2015 Elsevier Masson SAS。版权所有。
Kidney bean (Phaseolus vulgaris L.) is an important dietary legume crop cultivated and consumed worldwide. A purple cultivar (Zi Bawang) and a green cultivar (Chun Qiu), the main difference of which is in the pod skin color, were selected for the study. Malvidin 3, 5-diglucoside is identified as the major anthocyanin in the pod skin of Zi Bawang by HPLC-ESI-MS/MS. Three regulatory genes PvMYB1, PvMYB2, PvTT8-1 and most structural genes are dramatically up-regulated in purple pod skin compared to those in other materials. Significantly decreased expression levels of all regulatory genes and most biosynthetic genes are also detected in the purple skin of pods covered with bags compared to non-covered ones. All the results suggest that PvMYB1, PvMYB2 and PvTT8-1 might play a critical role in transcriptional activation of most anthocyanin biosynthetic genes in purple kidney bean pod. (C) 2015 Elsevier Masson SAS. All rights reserved.