Optimization of Cold-Active Lipase Production from Psychrophilic Bacterium Moritella sp 2-5-10-1 by Statistical Experimental Methods
Optimization of Cold-Active Lipase Production from Psychrophilic Bacterium Moritella sp 2-5-10-1 by Statistical Experimental Methods
复制标题
统计实验方法优化嗜冷菌 Moritella sp 2-5-10-1 生产冷活性脂肪酶
DOI：
10.1271/bbb.120104
复制
发表时间：
2013-01-01
影响因子：
1.6
通讯作者：
Guan, Xiangyu
中科院分区：
文献类型：
--
作者：
Wang, Quanfu;Zhang, Chunyu;Guan, Xiangyu
应用统计试验设计对嗜冷菌Moritella sp. 2-5-10-1产低温脂肪酶的工艺进行了优化。首先，使用Plackett-Burmen设计（PBD）来评估各种发酵参数的显著影响。结果表明，豆粕、温度和吐温-80对脂肪酶的产量有显著影响。随后使用中心复合设计（CCD）优化这些变量的水平。建立了低温活性脂肪酶生产的二次回归模型，并通过实验验证了模型的有效性。在优化的发酵条件下，在10 L的生物反应器中进行放大，获得了30.56 U/mL的低温活性脂肪酶产量。结果清楚地表明，该模型是足够的，即使在大规模。据我们所知，这是第一个报告的统计优化的冷活性脂肪酶生产的嗜冷细菌。
Statistical experimental designs were applied to optimize cold-active lipase production by the psychrophilic bacterium Moritella sp. 2-5-10-1. First, a Plackett-Burmen design (PBD) was used to evaluate the significant effects of various fermentation parameters. The results indicated that soybean meal, temperature, and Tween-80 had significant influences on lipase production. The levels of these variables were optimized subsequently using central composite design (CCD). A quadratic regression model of cold-active lipase production was built, and verification experiments confirmed its validity. On subsequent scale-up in a 10-L bioreactor using optimized conditions, cold-active lipase production (30.56 U/mL) was obtained. The results clearly indicated that the model was adequate even on a large scale. To our knowledge, this is the first report of statistical optimization of cold-active lipase production by a psychrophilic bacterium.