The coated pit and macropinocytic pathways serve distinct endosome populations.
The coated pit and macropinocytic pathways serve distinct endosome populations.
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DOI：
10.1083/jcb.124.5.689
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发表时间：
1994-03
期刊：
影响因子：
--
通讯作者：
Watts C
中科院分区：
文献类型：
--
作者：
Hewlett LJ;Prescott AR;Watts C
网格蛋白包被的囊泡内吞作用和巨胞吞作用是不同的内吞途径，在包括人表皮样A431细胞在内的几种细胞类型中都可以证明（West, m.a.， M.S. Bretscher, and C. Watts. 1989）。[j] .中国生物医学工程学报，2009(9):391 - 391。在这里，我们分析了巨量胞内体（巨量胞内体）含量与包被囊泡内吞作用的常规内体含量的混合程度。使用激光扫描共聚焦荧光显微镜，我们检测到很少的大肌醇体含量递送到早期或晚期的内体-溶酶体，用转铁蛋白或低密度脂蛋白标记。添加brefeldin A不会引起大肽体和常规内体的混合，而且在brefeldin A的存在下，大肽体的形态没有明显改变，而在相同的细胞中，常规内体出现了显着的管状效应。虽然难以与传统的内体融合，但巨小体是一种动态结构，有时在活细胞中表现出囊管状形态，并且能够相互融合。我们认为不同的内吞机制可以产生不同的内核体种群。
Clathrin-coated vesicle endocytosis and macropinocytosis are distinct endocytic pathways demonstrable in several cell types including human epidermoid A431 cells (West, M.A., M.S. Bretscher, and C. Watts. 1989. J. Cell Biol. 109:2731-2739). Here we analyze the extent of mixing of macropinocytic endosome (macropinosome) content with that of conventional endosomes served by coated vesicle endocytosis. Using laser scanning confocal fluorescence microscopy we detected very little delivery of macropinosome content to either early or late endosomes- lysosomes as defined by labeling with transferrin or with LDL. Mixing of the contents of the macropinosomes and conventional endosomes was not induced by the addition of brefeldin A. Moreover, the morphology of macropinosomes was not grossly altered in the presence of brefeldin A, whilst in the same cells there were dramatic tubulation effects on conventional endosomes as reported by others. Although refractory to fusion with conventional endosomes, macropinosomes were nonetheless dynamic structures which sometimes exhibited vesiculo-tubular morphology in living cells and were capable of fusing with each other. We suggest that different endocytic mechanisms can give rise to distinct endosome populations.