Apparent elimination of EIAV ancestral species in a long-term inapparent carrier
Apparent elimination of EIAV ancestral species in a long-term inapparent carrier
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DOI：
10.1016/j.virol.2005.09.006
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发表时间：
2006-01-20
期刊：
影响因子：
3.7
通讯作者：
Montelaro, RC
中科院分区：
文献类型：
--
作者：
Craigo, JK;Sturgeon, TJ;Montelaro, RC
马传染性贫血病毒（EIAV）包膜变异产生新的优势准种与每个连续的疾病周期，新的人口出现，和以前的血浆准种，包括原始接种，变得无法检测。问题仍然是这些祖先的变异是否存在于组织库中，或者免疫系统是否从持续感染中消除了准种。为了验证这一点，用地塞米松对EIAV长期隐性携带者进行免疫抑制。免疫抑制导致血浆病毒载量增加约10（4）倍。表征前和后免疫抑制人群表现出不断的信封演变，并揭示了新的准种不同的定义的人口从以前的疾病阶段。免疫抑制后的组织和血浆群体的分析表明，原始感染接种物和早期群体是不可检测的。因此，宿主免疫系统显然消除了各种抗原变体，但病毒持续存在是通过响应持续的免疫压力而从组织储库中无情地进化出新的包膜群体来维持的。(C)2005年爱思唯尔公司All rights reserved.
Equine infectious anemia virus (EIAV) envelope variation produces newly dominant quasispecies with each sequential disease cycle; new populations arise, and previous plasma quasispecies, including the original inoculum, become undetectable. The question remains whether these ancestral variants exist in tissue reservoirs or if the immune system eliminates quasispecies from persistent infections. To examine this, an EIAV long-term inapparent carrier was immune suppressed with dexamethasone. Immune suppression resulted in increased plasma viral loads by approximately 10(4) fold. Characterization of pre- and post-immune suppression populations demonstrated continual envelope evolution and revealed novel quasispecies distinct from defined populations from previous disease stages. Analysis of the tissue and plasma populations post-immune suppression indicated the original infectious inoculum and early populations were undetectable. Therefore, the host immune system apparently eliminated a diverse array of antigenic variants, but viral persistence was maintained by relentless evolution of new envelope populations from tissue reservoirs in response to ongoing immune pressures. (C) 2005 Elsevier Inc. All rights reserved.