A simple and effective coumarin-based fluorescent probe for cysteine
A simple and effective coumarin-based fluorescent probe for cysteine
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DOI：
10.1016/j.bios.2014.03.018
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发表时间：
2014-09-15
影响因子：
12.6
通讯作者：
Zhao, Bao-Xiang
中科院分区：
文献类型：
--
作者：
Dai, Xi;Wu, Qing-Hua;Zhao, Bao-Xiang
设计合成了一种简便、高效、高选择性、可裸眼检测的半胱氨酸荧光探针3-乙酰基-2-氧代-2H-色烯-7-基丙烯酸酯（ACA）。该探针可通过荧光光谱法检测半胱氨酸，检测限为0.657 μ M，可与小牛血清一起使用，并可用于活细胞成像。通过ESI-MS和荧光光谱证实了ACA与半胱氨酸反应的共轭加成/环化顺序机理。(C)2014 Elsevier BM. All rights reserved.
Acrylic acid 3-acetyl-2-oxo-2 H-chromen-7-yl ester (ACA) was rationally designed and synthesized as a simple and effective fluorescent probe for sensing cysteine with high selectivity and naked-eye detection. The probe can detect cysteine by fluorescence spectrometry with a detection limit of 0.657 mu M and can be used with calf serum and in live cell imaging. The conjugate addition/cyclization sequence mechanism of the reaction between ACA and cysteine was confirmed by ESI-MS and fluorescence spectra. (C) 2014 Elsevier BM. All rights reserved.