Bacterial characteristics and follicle surface structure: their roles in Peyer's patch uptake and transport of Vibrio cholerae.
Bacterial characteristics and follicle surface structure: their roles in Peyer's patch uptake and transport of Vibrio cholerae.
复制标题
细菌特征和滤泡表面结构：它们在派尔氏斑吸收和霍乱弧菌转运中的作用。
DOI：
10.1007/978-1-4684-5535-9_106
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发表时间：
1988
影响因子：
--
通讯作者：
Pierce,NF
中科院分区：
文献类型：
--
作者：
Owen,RL;CrayJr,WC;Ermak,TH;Pierce,NF
派尔集合淋巴结上的肠粘膜屏障的结构和功能特化促进与粘附的管腔颗粒和可溶性分子的接触和摄取。这些特化包括分泌粘液的杯状细胞的局部减少、淋巴滤泡对粘膜肌层的中断（可能改变粘膜运动性）以及M细胞的存在。M细胞是上皮细胞，具有不规则的、宽间隔的微绒毛、减少或不存在的溶酶体、活跃的跨细胞转运系统和通常被淋巴样细胞内陷的柔韧的基底外侧表面。M细胞在其管腔表面形成包被和未包被的囊泡，这些囊泡被转运到滤泡上皮细胞的细胞间隙，在那里发生肠抗原和淋巴样细胞的初始接触。横跨肠细胞（肠吸收细胞）微绒毛的粘液毯在M细胞上破裂，允许微生物和颗粒接近M细胞腔膜。标记凝集素的研究表明，M细胞膜上有一层薄薄的但可检测到的糖萼。这些凝集素检测到的糖包括β-D-半乳糖（蓖麻凝集素）、唾液酸和N-乙酰基-D-葡糖胺（麦胚凝集素），但不包括α-D-甘露糖或α-D-葡萄糖（Con A仅稀疏结合）。阳离子化铁蛋白均匀分布在M细胞腔膜上，表明存在阴离子位点。
Structural and functional specializations in the intestinal mucosal barrier over Peyer’s patches facilitate contact with and uptake of adherent luminal particles and soluble molecules. These specializations include local reduction in mucus-secreting goblet cells, interruption of the muscularis mucosae by lymphoid follicles(which may alter mucosal motility), and the presence of M cells. M cells are epithelial cells with irregular, widely spaced microvilli, reduced or absent lysosomes, an active transcellular transport system, and a pliable basolateral surface usually invaginated by lymphoid cells. At their luminal surfaces M cells form coated and uncoated vesicles, which are transported to intercellular spaces in the follicle epithelium where initial contact of intestinal antigen and lymphoid cells occurs. The mucus blanket which lies across the microvilli of enterocytes (intestinal absorptive cells) is broken over M cells, allowing microorganisms and particles to approach M cell luminal membranes. Studies with labeled lectins have demonstrated a thin but detectable glycocalyx coat on M cell membranes. Sugars detected by these lectins include beta-D-galactose (Ricinus communisagglutinin), sialic acid and N-acetyl-D-glucosamine (wheat germ agglutinin), but not alpha-D-mannose or alpha-D-glucose (Con A binds only sparsely). Cationized ferritin distributes evenly on M cell luminal membranes indicating that anionic sites are present.