Acetylcholine receptor antibody in myasthenia gravis: predominance of IgG subclasses 1 and 3.
Acetylcholine receptor antibody in myasthenia gravis: predominance of IgG subclasses 1 and 3.
复制标题
DOI：
--
复制
发表时间：
1987
影响因子：
4.6
通讯作者：
A. Rødgaard;F. Nielsen;R. Djurup;F. Somnier;S. Gammeltoft
中科院分区：
文献类型：
--
作者：
A. Rødgaard;F. Nielsen;R. Djurup;F. Somnier;S. Gammeltoft
用特异性免疫沉淀法测定了36例接受吡斯的明治疗的重症肌无力(MG)患者和8例胸腺切除患者血清中抗乙酰胆碱受体(AHR)的抗体水平。抗AchR亚类抗体IgG1、IgG2、IgG3和IgG4的阳性率分别为100%、33%、%和39%。按Osserman-Genkins分级标准，IgG1Ab浓度随病情加重而显著升高(rS=0.37，P<0.05)。36例接受吡斯的明治疗的MG患者的IgG1和IgG3亚类蛋白浓度显著高于44例年龄和性别匹配的健康受试者(P<0.0003)。胸腺切除后，2例患者的抗AchR IgG1浓度明显降低，而6例患者的AchR抗体水平无明显变化。这些结果支持这样一种假说，即抗AchR IgG1和Ig G3与神经肌肉接头上的AchR结合后，补体介导的细胞裂解或吞噬作用可能在MG的发病机制中起作用。
The concentrations of IgG subclass antibodies (Ab) to acetylcholine receptor (AchR) were quantified in 36 patients with myasthenia gravis (MG) treated with pyridostigmine only, and in eight patients who underwent thymectomy, using an IgG subclass-specific immunoprecipitation assay. IgG1, IgG2, IgG3, and IgG4 subclass Ab to AchR were present in 100%, 33%, 64% and 39% of the pyridostigmine-treated patients, respectively. The concentration of IgG1 Ab increased significantly with disease severity as graded by the Osserman-Genkins classification (rs = 0.37, P less than 0.05). IgG1 and IgG3 subclass protein concentrations were significantly higher (P less than 0.0003) in the 36 pyridostigmine-treated MG patients than in 44 age- and sex-matched healthy subjects. Thymectomy induced an appreciable reduction in anti-AchR IgG1 concentration in two patients, whereas six patients showed no changes in Ab to AchR. The results support the hypothesis that binding of anti-AchR IgG1 and IgG3 on AchR in the neuromuscular junction followed by complement-mediated cell lysis or phagocytosis, may play a role in the pathogenesis of MG.