Mucosal imbalance of interleukin-1β and interleukin-1 receptor antagonist in canine inflammatory bowel disease
Mucosal imbalance of interleukin-1β and interleukin-1 receptor antagonist in canine inflammatory bowel disease
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DOI：
10.1016/j.tvjl.2012.02.026
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发表时间：
2012-10-01
影响因子：
2.2
通讯作者：
Tsujimoto, Hajime
中科院分区：
文献类型：
--
作者：
Maeda, Shingo;Ohno, Koichi;Tsujimoto, Hajime
白细胞介素（IL）-1 β是炎症反应的关键介质。IL-1受体拮抗剂（IL-1 Ra）通过作为IL-1 β的内源性抑制剂发挥作用来调节炎症。在人类炎症性肠病（IBD）中已发现IL-1 β和IL-1 Ra之间平衡的破坏。本研究的目的是通过比较21只IBD犬、15只肠道淋巴瘤犬（“炎症”对照）和20只健康比格犬（“健康”对照）中IL-1 β和IL-1 Ra mRNA的表达（实时RT-PCR）和IL-1 β和IL-1 Ra蛋白的表达（ELISA），确定IBD犬中是否存在IL-1 β和IL-1 Ra的肠道失衡。与健康对照犬相比，在IBD病例中观察到肠IL-1 Ra：IL-1 β mRNA和蛋白的比例显著降低。相比之下，在患有肠淋巴瘤的犬中未观察到IL-1 Ra：IL-1 β比率的降低。IL-1 Ra：IL-1 β蛋白比值与IBD犬的临床严重程度呈负相关。IL-1 β和IL-1 Ra产生之间的肠道失衡可能在犬IBD的发病机制中起作用。(C)2012爱思唯尔有限公司版权所有。
Interleukin (IL)-1 beta is a key mediator of the inflammatory response. IL-1 receptor antagonist (IL-1Ra) regulates inflammation by functioning as an endogenous inhibitor of IL-1 beta. A disruption of the balance between IL-1 beta and IL-1Ra has been identified in human inflammatory bowel disease (IBD). The objective of this study was to determine whether there is an intestinal imbalance of IL-1 beta and IL-1Ra in canine IBD by comparing expression of IL-1 beta and IL-1Ra mRNA by real-time RT-PCR and expression of IL-1 beta and IL1Ra protein by ELISA in 21 dogs with IBD, 15 dogs with intestinal lymphoma ('inflammatory' controls) and 20 healthy Beagles ('healthy' controls). A significant decrease in the intestinal IL-1Ra:IL-1 beta ratio of mRNA and protein was observed in IBD cases when compared with healthy control dogs. In contrast, a decrease in IL-1Ra:IL-1 beta ratio was not observed in dogs with intestinal lymphoma. The IL-1Ra:IL-1 beta protein ratio was negatively correlated with clinical severity in dogs with IBD. An intestinal imbalance between IL-1 beta and IL-1Ra production may play a role in the pathogenesis of canine IBD. (C) 2012 Elsevier Ltd. All rights reserved.