Infection kinetics, prostacyclin release and cytokine-mediated modulation of the mechanism of cell death during bluetongue virus infection of cultured ovine and bovine pulmonary artery and lung microvascular endothelial cells
Infection kinetics, prostacyclin release and cytokine-mediated modulation of the mechanism of cell death during bluetongue virus infection of cultured ovine and bovine pulmonary artery and lung microvascular endothelial cells
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DOI：
10.1099/0022-1317-82-4-787
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发表时间：
2001-04-01
影响因子：
3.8
通讯作者：
MacLachlan, NJ
中科院分区：
文献类型：
--
作者：
DeMaula, CD;Jutila, MA;MacLachlan, NJ
蓝舌病病毒（BTV）感染引起绵羊出血性疾病，而BTV感染通常在牛中无症状。小血管内皮的损伤是BTV感染绵羊疾病表现的原因。肺是反刍动物BTV感染的发病机制的中心;因此，从绵羊和牛的肺动脉和肺微血管培养的内皮细胞（EC）被用于研究两个物种中蓝舌病不同表达的基础。用部分纯化的BTV以低多重性感染的绵羊和牛微血管EC对BTV诱导的细胞死亡同样敏感，但绵羊微血管EC的感染发生率较低，并且产生的病毒显著少于牛微血管EC。重要的是，凋亡细胞和坏死细胞的相对比例在BTV中有显著差异，感染的EC培养物取决于EC来源的物种和病毒接种物中炎性介质的存在。此外，BTV感染的绵羊肺微血管内皮细胞释放前列环素明显少于其他类型的内皮细胞。这些体外研究的结果与绵羊蓝舌病特征性的显著肺水肿和微血管血栓形成一致，但很少发生在BTV感染的牛中。
Bluetongue virus (BTV) infection causes a haemorrhagic disease in sheep, whereas BTV infection typically is asymptomatic in cattle. Injury to the endothelium of small blood vessels is responsible for the manifestations of disease in BTV-infected sheep. The lungs are central to the pathogenesis of BTV infection of ruminants; thus endothelial cells (ECs) cultured from the pulmonary artery and lung microvasculature of sheep and cattle were used to investigate the basis for the disparate expression of bluetongue disease in the two species. Ovine and bovine microvascular ECs infected at low multiplicity with partially purified BTV were equally susceptible to BTV-induced cell death, yet ovine microvascular ECs had a lower incidence of infection and produced significantly less virus than did bovine microvascular ECs, Importantly, the relative proportions of apoptotic and necrotic cells were significantly different in BTV-infected EC cultures depending on the species of EC origin and the presence of inflammatory mediators in the virus inoculum. Furthermore, BTV-infected ovine lung microvascular ECs released markedly less prostacyclin than the other types of ECs. Results of these in vitro studies are consistent with the marked pulmonary oedema and microvascular thrombosis that characterize bluetongue disease of sheep but which rarely, if ever, occur in BTV-infected cattle.