Cytomat-R: a computer-controlled multiple laser source multiparameter flow cytophotometer system.
Cytomat-R: a computer-controlled multiple laser source multiparameter flow cytophotometer system.
复制标题
Cytomat-R:计算机控制的多激光源多参数流式细胞光度计系统。
DOI:
10.1177/25.7.330733
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发表时间:
1977
期刊:
影响因子:
--
通讯作者:
M. Block
中科院分区:
文献类型:
--
作者:
H. Shapiro;E. Schildkraut;R. Curbelo;R. Turner;R. Webb;D. C. Brown;M. Block
A multiple illumination wavelength multiparameter flow cytophotometer system, using laser sources and controlled by a small, general-purpose digital computer, has been produced for use in the development of new flow cytometric techniques. Three different laser wave-lengths can be used simultaneously to illuminate different regions of the flow chamber; as many as five measurements of light scattering at various angles, extinction, and fluorescence at one or more wavelengths can be made at each illuminated station. Cells in suspension may be examined at rates of 1000 cells/sec, with seven correlated optical measurements being recorded for each cell. A library of programs for data manipulation and statistical analysis make it possible to use the system to develop and implement cell characterization, counting and classification procedures for basic and clinical research applications.